Human ACO1 ELISA Kit

Alternative name
Human Aconitase ELISA Kit;Human citrate hydro-lyase ELISA Kit;Human ferritin repressor protein ELISA Kit;Human iron regulatory protein 1 ELISA Kit;Human IRP1 ELISA Kit;Human iron-responsive element-binding protein 1 ELISA Kit;Human IRE-BP 1 ELISA Kit;Human IREB1 ELISA Kit;Human ACONS ELISA Kit;Human HEL60 ELISA Kit;Human IREBP ELISA Kit;Human IREBP1 ELISA Kit;Human aconitase 1 ELISA Kit;Human cytoplasmic aconitate hydratase ELISA Kit;Human aconitase 1, soluble ELISA Kit;Human aconitate hydratase, cytoplasmic ELISA Kit;Human cytosplasmic aconitase ELISA Kit;Human epididymis luminal protein 60 ELISA Kit;Human soluble aconitase ELISA Kit;
Catalog
E000342
species
Human
GeneACO1
Standard Curve
Other Species Mouse Aco1 ELISA Kit Bovine ACO1 ELISA Kit Chicken ACO1 ELISA Kit Rabbit ACO1 ELISA Kit Rat Aco1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Aconitase 1 (ACO1). No significant cross-reactivity or interference between Aconitase 1 (ACO1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.277ng/mL.
Intended UseHuman ACO1 ELISA Kit allows for the in vitro quantitative determination of ACO1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aconitase 1 (ACO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aconitase 1 (ACO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aconitase 1 (ACO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aconitase 1 (ACO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Cytoplasmic aconitate hydratase Protein information
Uniprot ID ACOC_HUMAN
Uniprot AC P21399; D3DRK7; Q14652; Q5VZA7;
UniGene Hs.567229;
GeneID 48
KEGG hsa:48;
Human Cytoplasmic aconitate hydratase Protein SEQUENCE
SEQUENCE 889 AA; 98399 MW; E1A05AF701D46DCB CRC64;
MSNPFAHLAE PLDPVQPGKK FFNLNKLEDS RYGRLPFSIR VLLEAAIRNC
DEFLVKKQDI ENILHWNVTQ HKNIEVPFKP ARVILQDFTG VPAVVDFAAM
RDAVKKLGGD PEKINPVCPA DLVIDHSIQV DFNRRADSLQ KNQDLEFERN
RERFEFLKWG SQAFHNMRII PPGSGIIHQV NLEYLARVVF DQDGYYYPDS
LVGTDSHTTM IDGLGILGWG VGGIEAEAVM LGQPISMVLP QVIGYRLMGK
PHPLVTSTDI VLTITKHLRQ VGVVGKFVEF FGPGVAQLSI ADRATIANMC
PEYGATAAFF PVDEVSITYL VQTGRDEEKL KYIKKYLQAV GMFRDFNDPS
QDPDFTQVVE LDLKTVVPCC SGPKRPQDKV AVSDMKKDFE SCLGAKQGFK
GFQVAPEHHN DHKTFIYDNT EFTLAHGSVV IAAITSCTNT SNPSVMLGAG
LLAKKAVDAG LNVMPYIKTS LSPGSGVVTY YLQESGVMPY LSQLGFDVVG
YGCMTCIGNS GPLPEPVVEA ITQGDLVAVG VLSGNRNFEG RVHPNTRANY
LASPPLVIAY AIAGTIRIDF EKEPLGVNAK GQQVFLKDIW PTRDEIQAVE
RQYVIPGMFK EVYQKIETVN ESWNALATPS DKLFFWNSKS TYIKSPPFFE
NLTLDLQPPK SIVDAYVLLN LGDSVTTDHI SPAGNIARNS PAARYLTNRG
LTPREFNSYG SRRGNDAVMA RGTFANIRLL NRFLNKQAPQ TIHLPSGEIL
DVFDAAERYQ QAGLPLIVLA GKEYGAGSSR DWAAKGPFLL GIKAVLAESY
ERIHRSNLVG MGVIPLEYLP GENADALGLT GQERYTIIIP ENLKPQMKVQ
VKLDTGKTFQ AVMRFDTDVE LTYFLNGGIL NYMIRKMAK

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Human ACO1 ELISA Kit