Human ACO1 ELISA Kit

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  • Alternative name

    Human Aconitase ELISA Kit;Human citrate hydro-lyase ELISA Kit;Human ferritin repressor protein ELISA Kit;Human iron regulatory protein 1 ELISA Kit;Human IRP1 ELISA Kit;Human iron-responsive element-binding protein 1 ELISA Kit;Human IRE-BP 1 ELISA Kit;Human IREB1 ELISA Kit;Human ACONS ELISA Kit;Human HEL60 ELISA Kit;Human IREBP ELISA Kit;Human IREBP1 ELISA Kit;Human aconitase 1 ELISA Kit;Human cytoplasmic aconitate hydratase ELISA Kit;Human aconitase 1, soluble ELISA Kit;Human aconitate hydratase, cytoplasmic ELISA Kit;Human cytosplasmic aconitase ELISA Kit;Human epididymis luminal protein 60 ELISA Kit;Human soluble aconitase ELISA Kit;

  • Catalog
    E000342
  • species
    Human
  • GeneACO1
  • Standard CurveHuman ACO1 ELISA Kit
  • Other Species Mouse Aco1 ELISA KitBovine ACO1 ELISA KitChicken ACO1 ELISA KitRabbit ACO1 ELISA KitRat Aco1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Aconitase 1 (ACO1). No significant cross-reactivity or interference between Aconitase 1 (ACO1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.277ng/mL.
  • Intended UseHuman ACO1 ELISA Kit allows for the in vitro quantitative determination of ACO1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aconitase 1 (ACO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aconitase 1 (ACO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aconitase 1 (ACO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aconitase 1 (ACO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Cytoplasmic aconitate hydratase Protein information
  • Uniprot ID ACOC_HUMAN
  • Uniprot AC P21399; D3DRK7; Q14652; Q5VZA7;
  • UniGene Hs.567229;
  • GeneID 48
  • KEGG hsa:48;
  • Human Cytoplasmic aconitate hydratase Protein SEQUENCE
  • SEQUENCE 889 AA; 98399 MW; E1A05AF701D46DCB CRC64;

    MSNPFAHLAE PLDPVQPGKK FFNLNKLEDS RYGRLPFSIR VLLEAAIRNC

    DEFLVKKQDI ENILHWNVTQ HKNIEVPFKP ARVILQDFTG VPAVVDFAAM

    RDAVKKLGGD PEKINPVCPA DLVIDHSIQV DFNRRADSLQ KNQDLEFERN

    RERFEFLKWG SQAFHNMRII PPGSGIIHQV NLEYLARVVF DQDGYYYPDS

    LVGTDSHTTM IDGLGILGWG VGGIEAEAVM LGQPISMVLP QVIGYRLMGK

    PHPLVTSTDI VLTITKHLRQ VGVVGKFVEF FGPGVAQLSI ADRATIANMC

    PEYGATAAFF PVDEVSITYL VQTGRDEEKL KYIKKYLQAV GMFRDFNDPS

    QDPDFTQVVE LDLKTVVPCC SGPKRPQDKV AVSDMKKDFE SCLGAKQGFK

    GFQVAPEHHN DHKTFIYDNT EFTLAHGSVV IAAITSCTNT SNPSVMLGAG

    LLAKKAVDAG LNVMPYIKTS LSPGSGVVTY YLQESGVMPY LSQLGFDVVG

    YGCMTCIGNS GPLPEPVVEA ITQGDLVAVG VLSGNRNFEG RVHPNTRANY

    LASPPLVIAY AIAGTIRIDF EKEPLGVNAK GQQVFLKDIW PTRDEIQAVE

    RQYVIPGMFK EVYQKIETVN ESWNALATPS DKLFFWNSKS TYIKSPPFFE

    NLTLDLQPPK SIVDAYVLLN LGDSVTTDHI SPAGNIARNS PAARYLTNRG

    LTPREFNSYG SRRGNDAVMA RGTFANIRLL NRFLNKQAPQ TIHLPSGEIL

    DVFDAAERYQ QAGLPLIVLA GKEYGAGSSR DWAAKGPFLL GIKAVLAESY

    ERIHRSNLVG MGVIPLEYLP GENADALGLT GQERYTIIIP ENLKPQMKVQ

    VKLDTGKTFQ AVMRFDTDVE LTYFLNGGIL NYMIRKMAK



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