Human INHBA ELISA Kit

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  • Alternative name

    Human activin beta-A chain ELISA Kit;Human erythroid differentiation protein ELISA Kit;Human EDF ELISA Kit;Human FRP ELISA Kit;Human inhibin beta A subunit ELISA Kit;Human inhibin beta A chain ELISA Kit;Human FSH-releasing protein ELISA Kit;Human Inhibin, beta-1 ELISA Kit;Human erythroid differentiation factor ELISA Kit;Human follicle-stimulating hormone-releasing protein ELISA Kit;Human inhibin, beta A (activin A, activin AB alpha polypeptide) ELISA Kit;

  • Catalog
    E000521
  • species
    Human
  • GeneINHBA
  • Other Species Mouse Inhba ELISA KitBovine INHBA ELISA KitChicken INHBA ELISA KitHorse INHBA ELISA KitPorcine INHBA ELISA KitRat Inhba ELISA KitSheep INHBA ELISA Kit
  • SpecificityNatural and recombinant human Activin A
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 12pg/ml
  • Intended UseHuman INHBA ELISA Kit allows for the in vitro quantitative determination of INHBA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The human Activin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Activin A has been precoated onto 96-well plates. Standards(CHO, G311-S426) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Activin A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Activin A amount of sample captured in plate. Background/Introduction: Activin A is a homodimer of 14kOa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. 1 Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types.2 Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
  • Human Inhibin beta A chain Protein information
  • Uniprot ID INHBA_HUMAN
  • Uniprot AC P08476; Q14599;
  • UniGene Hs.28792; Hs.583348;
  • GeneID 3624
  • KEGG hsa:3624;
  • Human Inhibin beta A chain Protein SEQUENCE
  • SEQUENCE 426 AA; 47442 MW; 201CDEDF99CB6919 CRC64;

    MPLLWLRGFL LASCWIIVRS SPTPGSEGHS AAPDCPSCAL AALPKDVPNS

    QPEMVEAVKK HILNMLHLKK RPDVTQPVPK AALLNAIRKL HVGKVGENGY

    VEIEDDIGRR AEMNELMEQT SEIITFAESG TARKTLHFEI SKEGSDLSVV

    ERAEVWLFLK VPKANRTRTK VTIRLFQQQK HPQGSLDTGE EAEEVGLKGE

    RSELLLSEKV VDARKSTWHV FPVSSSIQRL LDQGKSSLDV RIACEQCQES

    GASLVLLGKK KKKEEEGEGK KKGGGEGGAG ADEEKEQSHR PFLMLQARQS

    EDHPHRRRRR GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC

    EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS

    MLYYDDGQNI IKKDIQNMIV EECGCS

  • UCSC uc003thq.4; human.;




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