Human OGDH ELISA Kit

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  • Alternative name

    Human 2-oxoglutarate dehydrogenase complex component E1 ELISA Kit;Human OGDC-E1 ELISA Kit;Human Alpha-ketoglutarate dehydrogenase ELISA Kit;Human AKGDH ELISA Kit;Human E1k ELISA Kit;Human OGDC ELISA Kit;Human oxoglutarate dehydrogenase ELISA Kit;Human 2-oxoglutarate dehydrogenase, mitochondrial ELISA Kit;Human oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) ELISA Kit;Human oxoglutarate decarboxylase ELISA Kit;Human oxoglutarate dehydrogenase (succinyl-transferring) ELISA Kit;Human testicular tissue protein Li 131 ELISA Kit;

  • Catalog
    E027997
  • species
    Human
  • GeneOGDH
  • Standard CurveHuman OGDH ELISA Kit
  • Other Species Mouse Ogdh ELISA KitBovine OGDH ELISA KitMonkey OGDH ELISA KitRat Ogdh ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of OGDH. No significant cross-reactivity or interference between OGDH and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human OGDH is typically less than 0.59ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addin
  • Intended UseHuman OGDH ELISA Kit allows for the in vitro quantitative determination of OGDH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to OGDH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to OGDH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain OGDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of OGDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human 2-oxoglutarate dehydrogenase, mitochondrial Protein information
  • Uniprot ID ODO1_HUMAN
  • Uniprot AC Q02218; B4E2U9; D3DVL0; E9PBM1; Q96DD3; Q9UDX0;
  • UniGene Hs.488181;
  • GeneID 4967
  • KEGG hsa:4967;
  • Human 2-oxoglutarate dehydrogenase, mitochondrial Protein SEQUENCE
  • SEQUENCE 1023 AA; 115935 MW; F428DD342F232E7C CRC64;

    MFHLRTCAAK LRPLTASQTV KTFSQNRPAA ARTFQQIRCY SAPVAAEPFL

    SGTSSNYVEE MYCAWLENPK SVHKSWDIFF RNTNAGAPPG TAYQSPLPLS

    RGSLAAVAHA QSLVEAQPNV DKLVEDHLAV QSLIRAYQIR GHHVAQLDPL

    GILDADLDSS VPADIISSTD KLGFYGLDES DLDKVFHLPT TTFIGGQESA

    LPLREIIRRL EMAYCQHIGV EFMFINDLEQ CQWIRQKFET PGIMQFTNEE

    KRTLLARLVR STRFEEFLQR KWSSEKRFGL EGCEVLIPAL KTIIDKSSEN

    GVDYVIMGMP HRGRLNVLAN VIRKELEQIF CQFDSKLEAA DEGSGDVKYH

    LGMYHRRINR VTDRNITLSL VANPSHLEAA DPVVMGKTKA EQFYCGDTEG

    KKVMSILLHG DAAFAGQGIV YETFHLSDLP SYTTHGTVHV VVNNQIGFTT

    DPRMARSSPY PTDVARVVNA PIFHVNSDDP EAVMYVCKVA AEWRSTFHKD

    VVVDLVCYRR NGHNEMDEPM FTQPLMYKQI RKQKPVLQKY AELLVSQGVV

    NQPEYEEEIS KYDKICEEAF ARSKDEKILH IKHWLDSPWP GFFTLDGQPR

    SMSCPSTGLT EDILTHIGNV ASSVPVENFT IHGGLSRILK TRGEMVKNRT

    VDWALAEYMA FGSLLKEGIH IRLSGQDVER GTFSHRHHVL HDQNVDKRTC

    IPMNHLWPNQ APYTVCNSSL SEYGVLGFEL GFAMASPNAL VLWEAQFGDF

    HNTAQCIIDQ FICPGQAKWV RQNGIVLLLP HGMEGMGPEH SSARPERFLQ

    MCNDDPDVLP DLKEANFDIN QLYDCNWVVV NCSTPGNFFH VLRRQILLPF

    RKPLIIFTPK SLLRHPEARS SFDEMLPGTH FQRVIPEDGP AAQNPENVKR

    LLFCTGKVYY DLTRERKARD MVGQVAITRI EQLSPFPFDL LLKEVQKYPN

    AELAWCQEEH KNQGYYDYVK PRLRTTISRA KPVWYAGRDP AAAPATGNKK

    THLTELQRLL DTAFDLDVFK NFS

  • UCSC uc003tlm.4; human. [Q02218-1];


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