Human CTx2 ELISA Kit

Contact Us
  • Catalog
    E010234
  • species
    Human
  • GeneCTx2
  • Standard CurveHuman CTx2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CTX2. No significant cross-reactivity or interference between CTX2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CTX2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman CTx2 ELISA Kit allows for the in vitro quantitative determination of CTx2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Intended Uses: This CTX2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CTX2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CTX2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CTX2 antibody and an CTX2-HRP conjugate. The assay sample and buffer are incubated together with CTX2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CTX2 concentration since CTX2 from samples and CTX2-HRP conjugate compete for the anti-CTX2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CTX2 from the sample, fewer sites are left to bind CTX2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTX2 concentration in each sample is interpolated from this standard curve.


Related products with Human CTx2 ELISA Kit

Inquiry About Human CTx2 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!