Mouse F2 ELISA Kit

Alternative name
Mouse coagulation factor II ELISA Kit;Mouse Cf2 ELISA Kit;Mouse Cf-2 ELISA Kit;Mouse FII ELISA Kit;Mouse prothrombin ELISA Kit;Mouse thrombin ELISA Kit;
Catalog
E048761
species
Mouse
GeneF2
SpecificityThis assay has high sensitivity and excellent specificity for detection of F2. No significant cross-reactivity or interference between F2 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of mouse F2 is typically less than 0.59ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding
Intended UseMouse F2 ELISA Kit allows for the in vitro quantitative determination of F2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to F2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to F2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of F2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Thrombin heavy chain Protein information
Uniprot ID THRB_MOUSE
Uniprot AC P19221;
UniGene Mm.89048;
GeneID 14061
KEGG mmu:14061;
Mouse Thrombin heavy chain Protein SEQUENCE
SEQUENCE 618 AA; 70269 MW; B89F719AAFD601E0 CRC64;
MSHVRGLGLP GCLALAALVS LVHSQHVFLA PQQALSLLQR VRRANSGFLE
ELRKGNLERE CVEEQCSYEE AFEALESPQD TDVFWAKYTV CDSVRKPRET
FMDCLEGRCA MDLGVNYLGT VNVTHTGIQC QLWRSRYPHK PEINSTTHPG
ADLKENFCRN PDSSTTGPWC YTTDPTVRRE ECSVPVCGQE GRTTVVMTPR
SGGSKDNLSP PLGQCLTERG RLYQGNLAVT TLGSPCLPWN SLPAKTLSKY
QDFDPEVKLV ENFCRNPDWD EEGAWCYVAG QPGDFEYCNL NYCEEAVGEE
NYDVDESIAG RTTDAEFHTF FNEKTFGLGE ADCGLRPLFE KKSLKDTTEK
ELLDSYIDGR IVEGWDAEKG IAPWQVMLFR KSPQELLCGA SLISDRWVLT
AAHCILYPPW DKNFTENDLL VRIGKHSRTR YERNVEKISM LEKIYVHPRY
NWRENLDRDI ALLKLKKPVP FSDYIHPVCL PDKQTVTSLL RAGYKGRVTG
WGNLRETWTT NINEIQPSVL QVVNLPIVER PVCKASTRIR ITDNMFCAGF
KVNDTKRGDA CEGDSGGPFV MKSPFNNRWY QMGIVSWGEG CDRKGKYGFY
THVFRLKRWI QKVIDQFG
UCSC uc008kwg.2; mouse.;

Category

Mouse F2 ELISA Kit

Mouse coagulation factor II ELISA Kit;Mouse Cf2 ELISA Kit;Mouse Cf-2 ELISA Kit;Mouse FII ELISA Kit;Mouse prothrombin ELISA Kit;Mouse thrombin ELISA Kit;