Human ASL ELISA Kit

Alternative name
Human ASAL ELISA Kit;Human arginosuccinase ELISA Kit;Human argininosuccinate lyase ELISA Kit;Human argininosuccinase ELISA Kit;
Catalog
E003631
species
Human
GeneASL
Standard Curve
Other Species Mouse Asl ELISA Kit Bovine ASL ELISA Kit Monkey ASL ELISA Kit Rat Asl ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ASL. No significant cross-reactivity or interference between ASL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ASL and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman ASL ELISA Kit allows for the in vitro quantitative determination of ASL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This ASL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ASL. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ASL ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ASL antibody and an ASL -HRP conjugate. The assay sample and buffer are incubated together with ASL -HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ASL concentration since ASL from samples and ASL -HRP conjugate compete for the anti-ASL antibody binding site. Since the number of sites is limited, as more sites are occupied by ASL from the sample, fewer sites are left to bind ASL -HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASL concentration in each sample is interpolated from this standard curve.
Human Argininosuccinate lyase Protein information
Uniprot ID ARLY_HUMAN
Uniprot AC P04424; E7EMI0; E9PE48; Q6LDS5; Q96HS2;
UniGene Hs.632015;
GeneID 435
KEGG hsa:435;
Human Argininosuccinate lyase Protein SEQUENCE
SEQUENCE 464 AA; 51658 MW; F751625C1A581883 CRC64;
MASESGKLWG GRFVGAVDPI MEKFNASIAY DRHLWEVDVQ GSKAYSRGLE
KAGLLTKAEM DQILHGLDKV AEEWAQGTFK LNSNDEDIHT ANERRLKELI
GATAGKLHTG RSRNDQVVTD LRLWMRQTCS TLSGLLWELI RTMVDRAEAE
RDVLFPGYTH LQRAQPIRWS HWILSHAVAL TRDSERLLEV RKRINVLPLG
SGAIAGNPLG VDRELLRAEL NFGAITLNSM DATSERDFVA EFLFWASLCM
THLSRMAEDL ILYCTKEFSF VQLSDAYSTG SSLMPQKKNP DSLELIRSKA
GRVFGRCAGL LMTLKGLPST YNKDLQEDKE AVFEVSDTMS AVLQVATGVI
STLQIHQENM GQALSPDMLA TDLAYYLVRK GMPFRQAHEA SGKAVFMAET
KGVALNQLSL QELQTISPLF SGDVICVWDY GHSVEQYGAL GGTARSSVDW
QIRQVRALLQ AQQA
UCSC uc003tuo.4; human. [P04424-1];

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anti-ASL antibody

Category

Human ASL ELISA Kit

Human ASAL ELISA Kit;Human arginosuccinase ELISA Kit;Human argininosuccinate lyase ELISA Kit;Human argininosuccinase ELISA Kit;