Human ASL ELISA Kit

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  • Alternative name

    Human ASAL ELISA Kit;Human arginosuccinase ELISA Kit;Human argininosuccinate lyase ELISA Kit;Human argininosuccinase ELISA Kit;

  • Catalog
    E003631
  • species
    Human
  • GeneASL
  • Standard CurveHuman ASL ELISA Kit
  • Other Species Mouse Asl ELISA KitBovine ASL ELISA KitMonkey ASL ELISA KitRat Asl ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ASL. No significant cross-reactivity or interference between ASL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ASL and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ASL ELISA Kit allows for the in vitro quantitative determination of ASL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: This ASL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ASL. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ASL ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ASL antibody and an ASL -HRP conjugate. The assay sample and buffer are incubated together with ASL -HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ASL concentration since ASL from samples and ASL -HRP conjugate compete for the anti-ASL antibody binding site. Since the number of sites is limited, as more sites are occupied by ASL from the sample, fewer sites are left to bind ASL -HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASL concentration in each sample is interpolated from this standard curve.
  • Human Argininosuccinate lyase Protein information
  • Uniprot ID ARLY_HUMAN
  • Uniprot AC P04424; E7EMI0; E9PE48; Q6LDS5; Q96HS2;
  • UniGene Hs.632015;
  • GeneID 435
  • KEGG hsa:435;
  • Human Argininosuccinate lyase Protein SEQUENCE
  • SEQUENCE 464 AA; 51658 MW; F751625C1A581883 CRC64;

    MASESGKLWG GRFVGAVDPI MEKFNASIAY DRHLWEVDVQ GSKAYSRGLE

    KAGLLTKAEM DQILHGLDKV AEEWAQGTFK LNSNDEDIHT ANERRLKELI

    GATAGKLHTG RSRNDQVVTD LRLWMRQTCS TLSGLLWELI RTMVDRAEAE

    RDVLFPGYTH LQRAQPIRWS HWILSHAVAL TRDSERLLEV RKRINVLPLG

    SGAIAGNPLG VDRELLRAEL NFGAITLNSM DATSERDFVA EFLFWASLCM

    THLSRMAEDL ILYCTKEFSF VQLSDAYSTG SSLMPQKKNP DSLELIRSKA

    GRVFGRCAGL LMTLKGLPST YNKDLQEDKE AVFEVSDTMS AVLQVATGVI

    STLQIHQENM GQALSPDMLA TDLAYYLVRK GMPFRQAHEA SGKAVFMAET

    KGVALNQLSL QELQTISPLF SGDVICVWDY GHSVEQYGAL GGTARSSVDW

    QIRQVRALLQ AQQA

  • UCSC uc003tuo.4; human. [P04424-1];


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