Human IL4 ELISA Kit

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  • Alternative name

    Human IL-4 ELISA Kit;Human B-cell stimulatory factor 1 ELISA Kit;Human BSF-1 ELISA Kit;Human binetrakin ELISA Kit;Human lymphocyte stimulatory factor 1 ELISA Kit;Human pitrakinra ELISA Kit;Human BCGF-1 ELISA Kit;Human BCGF1 ELISA Kit;Human BSF1 ELISA Kit;Human interleukin 4 ELISA Kit;Human interleukin-4 ELISA Kit;Human B cell growth factor 1 ELISA Kit;Human B_cell stimulatory factor 1 ELISA Kit;Human interleukin 4 variant 2 ELISA Kit;

  • Catalog
    E019487
  • species
    Human
  • GeneIL4
  • Other Species Mouse Il4 ELISA KitBovine IL4 ELISA KitCanine IL4 ELISA KitGoat IL4 ELISA KitHorse IL4 ELISA KitMonkey IL4 ELISA KitPorcine IL4 ELISA KitRabbit IL4 ELISA KitRat Il4 ELISA KitSheep IL4 ELISA Kit
  • SpecificityThe Human IL-4 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-4 proteins within the range of 7.8 pg/ml - 500 pg/ml.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity2 pg/ml.
  • Intended UseHuman IL4 ELISA Kit allows for the in vitro quantitative determination of IL4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||The Human IL-4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-4 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Human IL-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.
  • Human Interleukin-4 Protein information
  • Uniprot ID IL4_HUMAN
  • Uniprot AC P05112; Q14630; Q6NZ77;
  • UniGene Hs.73917;
  • GeneID 3565
  • KEGG hsa:3565;
  • Human Interleukin-4 Protein SEQUENCE
  • SEQUENCE 153 AA; 17492 MW; 8725BF64B34D45F7 CRC64;

    MGLTSQLLPP LFFLLACAGN FVHGHKCDIT LQEIIKTLNS LTEQKTLCTE

    LTVTDIFAAS KNTTEKETFC RAATVLRQFY SHHEKDTRCL GATAQQFHRH

    KQLIRFLKRL DRNLWGLAGL NSCPVKEANQ STLENFLERL KTIMREKYSK

    CSS

  • UCSC uc003kxk.3; human. [P05112-1];



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