Human IL4 ELISA Kit

Alternative name
Human IL-4 ELISA Kit;Human B-cell stimulatory factor 1 ELISA Kit;Human BSF-1 ELISA Kit;Human binetrakin ELISA Kit;Human lymphocyte stimulatory factor 1 ELISA Kit;Human pitrakinra ELISA Kit;Human BCGF-1 ELISA Kit;Human BCGF1 ELISA Kit;Human BSF1 ELISA Kit;Human interleukin 4 ELISA Kit;Human interleukin-4 ELISA Kit;Human B cell growth factor 1 ELISA Kit;Human B_cell stimulatory factor 1 ELISA Kit;Human interleukin 4 variant 2 ELISA Kit;
Catalog
E019487
species
Human
GeneIL4
SpecificityThe Human IL-4 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-4 proteins within the range of 7.8 pg/ml - 500 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity2 pg/ml.
Intended UseHuman IL4 ELISA Kit allows for the in vitro quantitative determination of IL4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||The Human IL-4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-4 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Human IL-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.
Human Interleukin-4 Protein information
Uniprot ID IL4_HUMAN
Uniprot AC P05112; Q14630; Q6NZ77;
UniGene Hs.73917;
GeneID 3565
KEGG hsa:3565;
Human Interleukin-4 Protein SEQUENCE
SEQUENCE 153 AA; 17492 MW; 8725BF64B34D45F7 CRC64;
MGLTSQLLPP LFFLLACAGN FVHGHKCDIT LQEIIKTLNS LTEQKTLCTE
LTVTDIFAAS KNTTEKETFC RAATVLRQFY SHHEKDTRCL GATAQQFHRH
KQLIRFLKRL DRNLWGLAGL NSCPVKEANQ STLENFLERL KTIMREKYSK
CSS
UCSC uc003kxk.3; human. [P05112-1];

Human IL4 ELISA Kit

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