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Mouse Cd86 ELISA Kit

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  • Alternative name

    Mouse activation B7-2 antigen ELISA Kit;Mouse Early T-cell costimulatory molecule 1 ELISA Kit;Mouse ETC-1 ELISA Kit;Mouse B7 ELISA Kit;Mouse B7-2 ELISA Kit;Mouse B7.2 ELISA Kit;Mouse B70 ELISA Kit;Mouse CLS1 ELISA Kit;Mouse Cd28l2 ELISA Kit;Mouse Ly-58 ELISA Kit;Mouse Ly58 ELISA Kit;Mouse MB7 ELISA Kit;Mouse MB7-2 ELISA Kit;Mouse TS/A-2 ELISA Kit;Mouse CD86 antigen ELISA Kit;Mouse T-lymphocyte activation antigen CD86 ELISA Kit;Mouse early T cell costimulatory molecule-1 ELISA Kit;
  • Catalog
    E047778
  • species
    Mouse
  • GeneCd86
  • SpecificityThe Mouse CD86 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse CD86 proteins within the range of 62.5 pg/ml - 4000 pg/ml.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of Mouse CD86 is typically less than 10 pg/ml.
  • Intended UseMouse Cd86 ELISA Kit allows for the in vitro quantitative determination of Cd86 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Background/Introduction: Cluster of Differentiation 86 (also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma. Principle of the Assay: The Bioscience Mouse CD86 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD86 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse CD86 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD86 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD86 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD86 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
  • Mouse T-lymphocyte activation antigen CD86 Protein information
  • Uniprot ID CD86_MOUSE
  • Uniprot AC P42082;
  • UniGene Mm.1452;
  • GeneID 12524
  • KEGG mmu:12524;
  • Mouse T-lymphocyte activation antigen CD86 Protein SEQUENCE

  • SEQUENCE 309 AA; 34666 MW; 8F58DCD1FB81D5EA CRC64;

    MDPRCTMGLA ILIFVTVLLI SDAVSVETQA YFNGTAYLPC PFTKAQNISL

    SELVVFWQDQ QKLVLYEHYL GTEKLDSVNA KYLGRTSFDR NNWTLRLHNV

    QIKDMGSYDC FIQKKPPTGS IILQQTLTEL SVIANFSEPE IKLAQNVTGN

    SGINLTCTSK QGHPKPKKMY FLITNSTNEY GDNMQISQDN VTELFSISNS

    LSLSFPDGVW HMTVVCVLET ESMKISSKPL NFTQEFPSPQ TYWKEITASV

    TVALLLVMLL IIVCHKKPNQ PSRPSNTASK LERDSNADRE TINLKELEPQ

    IASAKPNAE

  • UCSC uc007zcq.2; mouse. [P42082-1];



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