SpecificityThis assay has high sensitivity and excellent specificity for detection of ANAb. No significant cross-reactivity or interference between ANAb and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANAb and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman ANA ELISA Kit allows for the in vitro quantitative determination of ANA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses: This ANAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ANAb. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||ANAb ELISA kit applies the competitive enzyme immunoassay technique utilizing Nuclear antigen and an ANAb-HRP conjugate. The assay sample and buffer are incubated together with ANAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANAb concentration since ANAb from samples and ANAb-HRP conjugate compete for the Nuclear antigen binding site. Since the number of sites is limited, as more sites are occupied by ANAb from the sample, fewer sites are left to bind ANAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANAb concentration in each sample is interpolated from this standard curve.