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Alternative name
Inhibitor of nuclear factor kappa-B kinase subunit alpha ELISA KIT; Conserved helix-loop-helix ubiquitous kinase ELISA KIT; I-kappa-B kinase 1 ELISA KIT; IKK1 ELISA KIT; Nuclear factor NF-kappa-B inhibitor kinase alpha ELISA KIT; NFKBIKA ELISA KIT; Transcription factor 16 ELISA KIT; TCF-16CHUK ELISA KIT; IKKA ELISA KIT; TCF16 ELISA KIT; I-kappa-B kinase alpha ELISA KIT; IKK-A ELISA KIT; IKK-alpha ELISA KIT; IkBKA ELISA KIT; IkappaB kinase ELISA KIT; IKK1 ELISA KIT; NFKBIKA ELISA KIT; TCF-16 ELISA KIT
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Catalog
E056645
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species
Mouse
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GeneIkBa
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Inhibitory Subunit Of NF Kappa B Alpha (IkBa).
No significant cross-reactivity or interference between Inhibitory Subunit Of NF Kappa B Alpha (IkBa) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.059ng/mL.
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Intended UseMouse IkBa ELISA Kit allows for the in vitro quantitative determination of IkBa , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inhibitory Subunit Of NF Kappa B Alpha (IkBa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inhibitory Subunit Of NF Kappa B Alpha (IkBa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inhibitory Subunit Of NF Kappa B Alpha (IkBa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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