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Mouse Cd70 ELISA Kit

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  • Alternative name

    Mouse CD27 ligand ELISA Kit;Mouse CD27-L ELISA Kit;Mouse tumor necrosis factor ligand superfamily member 7 ELISA Kit;Mouse Cd27l ELISA Kit;Mouse CD27LG ELISA Kit;Mouse Tnfsf7 ELISA Kit;Mouse Tnlg8a ELISA Kit;Mouse CD70 antigen ELISA Kit;Mouse Ki-24 antigen ELISA Kit;Mouse tumor necrosis factor ligand 8a ELISA Kit;
  • Catalog
    E047759
  • species
    Mouse
  • GeneCd70
  • SpecificityThe Mouse CD70 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse CD70 proteins within the range of 31.2 pg/ml - 2000 pg/ml.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity10 pg/ml.
  • Intended UseMouse Cd70 ELISA Kit allows for the in vitro quantitative determination of Cd70 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||The Mouse CD70 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD70 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse CD70 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD70 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD70 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD70 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: CD70 (Cluster of Differentiation 70), also known as TNFSF7 or CD27L, is a ligand for CD27. This gene is mapped to 19p13.3. CD70 is a surface antigen found on activated, but not resting, T and B lymphocytes. It has been found that CD70 induced proliferation of costimulated T cells and enhanced generation of cytolytic T cells. CD70 is homologous to the ligands of the TNF receptor family, including TNF-alpha, TNF-beta, and the CD40 ligand, showing 19 to 24% amino acid sequence identity in the extracellular region.
  • Mouse CD70 antigen Protein information
  • Uniprot ID CD70_MOUSE
  • Uniprot AC O55237;
  • UniGene Mm.42228;
  • GeneID 21948
  • KEGG mmu:21948;
  • Mouse CD70 antigen Protein SEQUENCE

  • SEQUENCE 195 AA; 21919 MW; BD14671A2C8E26C7 CRC64;

    MPEEGRPCPW VRWSGTAFQR QWPWLLLVVF ITVFCCWFHC SGLLSKQQQR

    LLEHPEPHTA ELQLNLTVPR KDPTLRWGAG PALGRSFTHG PELEEGHLRI

    HQDGLYRLHI QVTLANCSSP GSTLQHRATL AVGICSPAAH GISLLRGRFG

    QDCTVALQRL TYLVHGDVLC TNLTLPLLPS RNADETFFGV QWICP

  • UCSC uc008dee.1; mouse.;



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