Intended UseHuman AMA (IgG) ELISA Kit allows for the in vitro quantitative determination of AMA (IgG) , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with myocardial antigen. Sample Diluent and Samples are then added to the appropriate microtiter plate wells and incubated. Then add Horseradish Peroxidase (HRP)-conjugated anti-human IgG and incubated. Then substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of anti-myocardial antibody AMA in the samples is then determined by comparing the O.D. of the samples to the standard curve.