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Human GLO1 ELISA Kit

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  • Alternative name

    Human aldoketomutase ELISA Kit;Human glyoxalase I ELISA Kit;Human glx I ELISA Kit;Human ketone-aldehyde mutase ELISA Kit;Human methylglyoxalase ELISA Kit;Human S-D-lactoylglutathione methylglyoxal lyase ELISA Kit;Human GLOD1 ELISA Kit;Human GLYI ELISA Kit;Human HEL-S-74 ELISA Kit;Human lactoylglutathione lyase ELISA Kit;Human epididymis secretory protein Li 74 ELISA Kit;Human glyoxalase domain containing 1 ELISA Kit;Human lactoyl glutathione lyase ELISA Kit;
  • Catalog
    E017208
  • species
    Human
  • GeneGLO1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Glyoxalase I (GLO1). No significant cross-reactivity or interference between Glyoxalase I (GLO1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.54ng/mL.
  • Intended UseHuman GLO1 ELISA Kit allows for the in vitro quantitative determination of GLO1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glyoxalase I (GLO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glyoxalase I (GLO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glyoxalase I (GLO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glyoxalase I (GLO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Lactoylglutathione lyase Protein information
  • Uniprot ID LGUL_HUMAN
  • Uniprot AC Q04760; B2R6P7; B4DDV0; P78375; Q59EL0; Q5TZW3; Q96FC0; Q96J41;
  • UniGene Hs.268849;
  • GeneID 2739
  • KEGG hsa:2739;
  • Human Lactoylglutathione lyase Protein SEQUENCE

  • SEQUENCE 184 AA; 20778 MW; 46291B7878070028 CRC64;

    MAEPQPPSGG LTDEAALSCC SDADPSTKDF LLQQTMLRVK DPKKSLDFYT

    RVLGMTLIQK CDFPIMKFSL YFLAYEDKND IPKEKDEKIA WALSRKATLE

    LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV

    KKPDDGKMKG LAFIQDPDGY WIEILNPNKM ATLM




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