Mouse Acer1 ELISA Kit

Alternative name
Mouse alkCDase 1 ELISA Kit;Mouse alkaline cdase 1 ELISA Kit;Mouse maCER1 ELISA Kit;Mouse acylsphingosine deacylase 3 ELISA Kit;Mouse N-acylsphingosine amidohydrolase 3 ELISA Kit;Mouse Asah3 ELISA Kit;Mouse 2310024P18Rik ELISA Kit;Mouse AI662009 ELISA Kit;Mouse Alkcdase1 ELISA Kit;Mouse Cer1 ELISA Kit;Mouse alkaline ceramidase 1 ELISA Kit;
Catalog
E043228
species
Mouse
GeneAcer1
SpecificityThis assay has high sensitivity and excellent specificity for detection of ACER1. No significant cross-reactivity or interference between ACER1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACER1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Acer1 ELISA Kit allows for the in vitro quantitative determination of Acer1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: ACER1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACER1 antibody and an ACER1-HRP conjugate. The assay sample and buffer are incubated together with ACER1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACER1 concentration since ACER1 from samples and ACER1-HRP conjugate compete for the anti-ACER1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACER1 from the sample, fewer sites are left to bind ACER1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACER1 concentration in each sample is interpolated from this standard curve.
Mouse Alkaline ceramidase 1 Protein information
Uniprot ID ACER1_MOUSE
Uniprot AC Q8R4X1; A1L3S2;
UniGene Mm.218784;
GeneID 171168
KEGG mmu:171168;
Mouse Alkaline ceramidase 1 Protein SEQUENCE
SEQUENCE 273 AA; 32082 MW; 0B84D015D7870219 CRC64;
MHVPGTRAKM SSIFAYQSSE VDWCESNFQH SELVAEFYNT FSNVFFLIFG
PLMMFLMHPY AQKRTRCFYG VSVLFMLIGL FSMYFHMTLS FLGQLLDEIS
ILWLLASGYS VWLPRCYFPK FVKGNRFYFS CLVTITTIIS TFLTFVKPTV
NAYALNSIAI HILYIVRTEY KKIRDDDLRH LIAVSVVLWA AALTSWISDR
VLCSFWQRIH FYYLHSIWHV LISITFPYGI VTMALVDAKY EMPDKTLKVH
YWPRDSWVIG LPYVEIQEND KNC
UCSC uc008ddl.1; mouse.;

Mouse Acer1 ELISA Kit

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