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Mouse Cited2 ELISA Kit

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  • Alternative name

    Mouse MSG-related protein 1 ELISA Kit;Mouse MRG-1 ELISA Kit;Mouse p35srj ELISA Kit;Mouse Mrg1 ELISA Kit;Mouse Msg2 ELISA Kit;Mouse AI835299 ELISA Kit;Mouse ER154-like ELISA Kit;Mouse Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2 ELISA Kit;Mouse cbp/p300-interacting transactivator 2 ELISA Kit;Mouse melanocyte specific gene 2 ELISA Kit;
  • Catalog
    E047502
  • species
    Mouse
  • GeneCited2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CITED2. No significant cross-reactivity or interference between CITED2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CITED2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Cited2 ELISA Kit allows for the in vitro quantitative determination of Cited2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical    Intended Uses: This CITED2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CITED2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CITED2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CITED2 antibody and an CITED2-HRP conjugate. The assay sample and buffer are incubated together with CITED2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CITED2 concentration since CITED2 from samples and CITED2-HRP conjugate compete for the anti-CITED2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CITED2 from the sample, fewer sites are left to bind CITED2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CITED2 concentration in each sample is interpolated from this standard curve.
  • Mouse Cbp/p300-interacting transactivator 2 Protein information
  • Uniprot ID CITE2_MOUSE
  • Uniprot AC O35740; O35741; O35742; O35743; O55198; Q6PGA9;
  • UniGene Mm.272321;
  • GeneID 17684
  • KEGG mmu:17684;
  • Mouse Cbp/p300-interacting transactivator 2 Protein SEQUENCE

  • SEQUENCE 269 AA; 28321 MW; 0FFCD8C21E4E3D81 CRC64;

    MADHMMAMNH GRFPDGTNGL HHHPAHRMGM GQFPSPHHHQ QQQPQHAFNA

    LMGEHIHYGA GNMNATSGIR HAMGPGTVNG GHPPSALAPA ARFNNSQFMG

    PPVASQGGSL PASMQLQKLN NQYFNHHPYP HNHYMPDLHP TAGHQMNGTN

    QHFRDCNPKH SGGSSTPGGA GGSGTPGGSG GTSGGAGGSS AGGSGGGSTM

    PASVAHVPAA MLPPNVIDTD FIDEEVLMSL VIEMGLDRIK ELPELWLGQN

    EFDFMTDFVC KQQPSRVSC

  • UCSC uc007elt.2; mouse. [O35740-1];



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