Human y+LAT1 ELISA Kit

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  • Alternative name

    Large neutral amino acids transporter small subunit 1 ELISA KIT; 4F2 light chain ELISA KIT; 4F2 LC ELISA KIT; 4F2LC ELISA KIT; CD98 light chain ELISA KIT; Integral membrane protein E16 ELISA KIT; L-type amino acid transporter 1 ELISA KIT; hLAT1 ELISA KIT; Solute carrier family 7 member 5 ELISA KIT; y+ system cationic amino acid transporterSLC7A5 ELISA KIT; CD98LC ELISA KIT; LAT1 ELISA KIT; MPE16 ELISA KIT; 4F2 LC ELISA KIT; 4F2LC ELISA KIT; hLAT1 ELISA KIT

  • Catalog
    E022036
  • species
    Human
  • Geney+LAT1
  • Standard CurveHuman y+LAT1 ELISA Kit
  • Other Species Mouse y+LAT1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of L-Type Amino Acid Transporter 1 (y+LAT1). No significant cross-reactivity or interference between L-Type Amino Acid Transporter 1 (y+LAT1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.114ng/mL.
  • Intended UseHuman y+LAT1 ELISA Kit allows for the in vitro quantitative determination of y+LAT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to L-Type Amino Acid Transporter 1 (y+LAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to L-Type Amino Acid Transporter 1 (y+LAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain L-Type Amino Acid Transporter 1 (y+LAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of L-Type Amino Acid Transporter 1 (y+LAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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