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Human ETFB ELISA Kit

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  • Alternative name

    Human beta-ETF ELISA Kit;Human FP585 ELISA Kit;Human MADD ELISA Kit;Human electron transfer flavoprotein beta subunit ELISA Kit;Human electron transfer flavoprotein subunit beta ELISA Kit;Human electron transfer flavoprotein, beta polypeptide ELISA Kit;Human electron-transferring-flavoprotein, beta polypeptide ELISA Kit;
  • Catalog
    E012907
  • species
    Human
  • GeneETFB
  • Standard CurveHuman ETFB ELISA Kit
  • Other Species Mouse Etfb ELISA KitBovine ETFB ELISA KitPorcine ETFB ELISA KitRat Etfb ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Electron Transfer Flavoprotein Beta Polypeptide (ETFb). No significant cross-reactivity or interference between Electron Transfer Flavoprotein Beta Polypeptide (ETFb) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.112ng/mL.
  • Intended UseHuman ETFB ELISA Kit allows for the in vitro quantitative determination of ETFB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Electron Transfer Flavoprotein Beta Polypeptide (ETFb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Electron Transfer Flavoprotein Beta Polypeptide (ETFb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Electron Transfer Flavoprotein Beta Polypeptide (ETFb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Electron Transfer Flavoprotein Beta Polypeptide (ETFb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Electron transfer flavoprotein subunit beta Protein information
  • Uniprot ID ETFB_HUMAN
  • Uniprot AC P38117; A8K766; B3KNY2; Q6IBH7; Q71RF6; Q9Y3S7;
  • UniGene Hs.348531;
  • GeneID 2109
  • KEGG hsa:2109;
  • Human Electron transfer flavoprotein subunit beta Protein SEQUENCE

  • SEQUENCE 255 AA; 27844 MW; 47E6EAEF50EB2C80 CRC64;

    MAELRVLVAV KRVIDYAVKI RVKPDRTGVV TDGVKHSMNP FCEIAVEEAV

    RLKEKKLVKE VIAVSCGPAQ CQETIRTALA MGADRGIHVE VPPAEAERLG

    PLQVARVLAK LAEKEKVDLV LLGKQAIDDD CNQTGQMTAG FLDWPQGTFA

    SQVTLEGDKL KVEREIDGGL ETLRLKLPAV VTADLRLNEP RYATLPNIMK

    AKKKKIEVIK PGDLGVDLTS KLSVISVEDP PQRTAGVKVE TTEDLVAKLK

    EIGRI

  • UCSC uc002pwg.4; human. [P38117-1];


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