Mouse Casp3 ELISA Kit

Alternative name
Mouse CASP-3 ELISA Kit;Mouse apopain ELISA Kit;Mouse cysteine protease CPP32 ELISA Kit;Mouse CPP-32 ELISA Kit;Mouse Lice ELISA Kit;Mouse protein Yama ELISA Kit;Mouse SREBP cleavage activity 1 ELISA Kit;Mouse SCA-1 ELISA Kit;Mouse CPP32 ELISA Kit;Mouse A830040C14Rik ELISA Kit;Mouse AC-3 ELISA Kit;Mouse CC3 ELISA Kit;Mouse caspase-3 ELISA Kit;Mouse Yama ELISA Kit;Mouse mldy ELISA Kit;Mouse caspase 3 ELISA Kit;Mouse caspase 3, apoptosis related cysteine protease ELISA Kit;
Catalog
E047235
species
Mouse
GeneCasp3
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse CASP3. No significant cross-reactivity or interference between Mouse CASP3 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CASP3 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.094ng/mL
Intended UseMouse Casp3 ELISA Kit allows for the in vitro quantitative determination of Casp3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CASP3 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CASP3. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CASP3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CASP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CASP3. You can calculate the concentration of Mouse CASP3 in the samples by comparing the OD of the samples to the standard curve.
Mouse Caspase-3 subunit p12 Protein information
Uniprot ID CASP3_MOUSE
Uniprot AC P70677; O08668; Q8CHV5; Q9QWI4;
UniGene Mm.34405;
GeneID 12367
KEGG mmu:12367;
Mouse Caspase-3 subunit p12 Protein SEQUENCE
SEQUENCE 277 AA; 31475 MW; CE91598F74826605 CRC64;
MENNKTSVDS KSINNFEVKT IHGSKSVDSG IYLDSSYKMD YPEMGICIII
NNKNFHKSTG MSSRSGTDVD AANLRETFMG LKYQVRNKND LTREDILELM
DSVSKEDHSK RSSFVCVILS HGDEGVIYGT NGPVELKKLT SFFRGDYCRS
LTGKPKLFII QACRGTELDC GIETDSGTDE EMACQKIPVE ADFLYAYSTA
PGYYSWRNSK DGSWFIQSLC SMLKLYAHKL EFMHILTRVN RKVATEFESF
SLDSTFHAKK QIPCIVSMLT KELYFYH
UCSC uc009lql.2; mouse.;

Mouse Casp3 ELISA Kit

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