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Catalog
E002135
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species
Human
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GeneAT-1
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Standard Curve
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Other Species
Mouse AT-1 ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Ang2R1Ab. No significant cross-reactivity or interference between Ang2R1Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Ang2R1Ab and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 ng/mL.
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Intended UseHuman AT-1 ELISA Kit allows for the in vitro quantitative determination of AT-1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyHuman ELISA Kit
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Product Description
specificalPrinciple of the assay: Ang2R1Ab ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with Angiotensin 2 Receptor 1 antigen. Standards or samples are then added to the microtiter plate wells and Ang2R1Ab if present, will bind to the antigen pre-coated wells. In order to quantitatively determine the amount of Ang2R1Ab present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated Angiotensin 2 Receptor 1 antibody is added to each well to "sandwich" the Ang2R1Ab immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Ang2R1Ab and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Ang2R1Ab concentration in each sample is interpolated from this standard curve.
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