Mouse ALP ELISA Kit

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  • Alternative name

    Alkaline phosphatase, tissue-nonspecific isozyme ELISA KIT; Alkaline phosphatase liver/bone/kidney isozymeALPL ELISA KIT; AP-TNAP ELISA KIT; TNSALP ELISA KIT

  • Catalog
    E043233
  • species
    Mouse
  • GeneALP
  • Other Species Human ALP ELISA KitHuman ALP-B ELISA KitMouse ALP-B ELISA Kit
  • SpecificityNo cross-reaction with other factors.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.5ng/ml.
  • Intended UseMouse ALP ELISA Kit allows for the in vitro quantitative determination of ALP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse ALP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.



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