Mouse Bmp2 ELISA Kit

Alternative name
Mouse Bmp-2 ELISA Kit;Mouse bone morphogenetic protein 2A ELISA Kit;Mouse BMP-2A ELISA Kit;Mouse AI467020 ELISA Kit;Mouse Bmp2a ELISA Kit;Mouse bone morphogenetic protein 2 ELISA Kit;
Catalog
E045919
species
Mouse
GeneBmp2
Standard Curve
Other Species Human BMP2 ELISA Kit Chicken BMP2 ELISA Kit Rabbit BMP2 ELISA Kit Rat Bmp2 ELISA Kit
SpecificityNatural and recombinant mouse BMP-2
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 2pg/ml
Intended UseMouse Bmp2 ELISA Kit allows for the in vitro quantitative determination of Bmp2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: mouse BMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BMP-2 has been precoated onto 96-well plates. Standards(CHO, Q283-R396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BMP-2 amount of sample captured in plate. Background: Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein inducesmedulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinicaltrials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant mouse BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.
Mouse Bone morphogenetic protein 2 Protein information
Uniprot ID BMP2_MOUSE
Uniprot AC P21274; Q497W8;
UniGene Mm.103205;
GeneID 12156
KEGG mmu:12156;
Mouse Bone morphogenetic protein 2 Protein SEQUENCE
SEQUENCE 394 AA; 44525 MW; D0C03B63B1D5C4E2 CRC64;
MVAGTRCLLV LLLPQVLLGG AAGLIPELGR KKFAAASSRP LSRPSEDVLS
EFELRLLSMF GLKQRPTPSK DVVVPPYMLD LYRRHSGQPG APAPDHRLER
AASRANTVRS FHHEEAVEEL PEMSGKTARR FFFNLSSVPS DEFLTSAELQ
IFREQIQEAL GNSSFQHRIN IYEIIKPAAA NLKFPVTRLL DTRLVNQNTS
QWESFDVTPA VMRWTTQGHT NHGFVVEVAH LEENPGVSKR HVRISRSLHQ
DEHSWSQIRP LLVTFGHDGK GHPLHKREKR QAKHKQRKRL KSSCKRHPLY
VDFSDVGWND WIVAPPGYHA FYCHGECPFP LADHLNSTNH AIVQTLVNSV
NSKIPKACCV PTELSAISML YLDENEKVVL KNYQDMVVEG CGCR
UCSC uc008mnr.2; mouse.;

Related products with Mouse Bmp2 ELISA Kit

Category

Mouse Bmp2 ELISA Kit

Mouse Bmp-2 ELISA Kit;Mouse bone morphogenetic protein 2A ELISA Kit;Mouse BMP-2A ELISA Kit;Mouse AI467020 ELISA Kit;Mouse Bmp2a ELISA Kit;Mouse bone morphogenetic protein 2 ELISA Kit;