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Human RNP ELISA Kit

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  • Alternative name

    Heterogeneous nuclear ribonucleoprotein M ELISA KIT; HNRNPM ELISA KIT; HNRPM ELISA KIT; NAGR1 ELISA KIT; hnRNP M ELISA KIT
  • Catalog
    E002676
  • species
    Human
  • GeneRNP
  • Standard CurveHuman RNP ELISA Kit
  • Other Species Human RNP-Ab ELISA KitHuman RNP-70 ELISA KitHuman RNP/Sm ELISA KitHuman RNP/Sm IgG ELISA KitMouse RNP ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 U/mL.
  • Intended UseHuman RNP ELISA Kit allows for the in vitro quantitative determination of RNP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical    Principle of the Assay: Anti-RNP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Anti-RNP. Standards or samples are then added to the microtiter plate wells and Anti-RNP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Anti-RNP present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for Anti-RNP are added to each well to "sandwich" the Anti-RNP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Anti-RNP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-RNP concentration in each sample is interpolated from this standard curve.


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