SensitivityThe minimum detectable dose of human NENF is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman NENF ELISA Kit allows for the in vitro quantitative determination of NENF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NENF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NENF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NENF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NENF bound in the initial step. The color development is stopped and the intensity of the color is measured.