Human cell immortalization-related protein 2 ELISA Kit;Human neuron-derived neurotrophic factor ELISA Kit;Human Protein GIG47 ELISA Kit;Human secreted protein of unknown function ELISA Kit;Human SPUF protein ELISA Kit;Human CIR2 ELISA Kit;Human SPUF ELISA Kit;Human SCIRP10 ELISA Kit;Human neudesin neurotrophic factor ELISA Kit;Human neudesin ELISA Kit;Human SCIRP10-related protein ELISA Kit;Human Spinal cord injury related protein 10 ELISA Kit;Human cell growth-inhibiting protein 47 ELISA Kit;
Catalog
E026241
species
Human
GeneNENF
SpecificityThis assay has high sensitivity and excellent specificity for detection of human NENF. No significant cross-reactivity or interference between human NENF and analogues was observed.
SensitivityThe minimum detectable dose of human NENF is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman NENF ELISA Kit allows for the in vitro quantitative determination of NENF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NENF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NENF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NENF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NENF bound in the initial step. The color development is stopped and the intensity of the color is measured.