Human BAX ELISA Kit

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  • Alternative name

    Human bcl-2-like protein 4 ELISA Kit;Human bcl2-L-4 ELISA Kit;Human BCL2L4 ELISA Kit;Human BCL2 associated X, apoptosis regulator ELISA Kit;Human apoptosis regulator BAX ELISA Kit;Human BCL2 associated X protein ELISA Kit;Human BCL2-associated X protein omega ELISA Kit;Human Baxdelta2G9 ELISA Kit;Human Baxdelta2G9omega ELISA Kit;Human Baxdelta2omega ELISA Kit;

  • Catalog
    E004558
  • species
    Human
  • GeneBAX
  • Standard CurveHuman BAX ELISA Kit
  • Other Species Mouse Bax ELISA KitBovine BAX ELISA KitRat Bax ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Bcl2 Associated X Protein (Bax). No significant cross-reactivity or interference between Bcl2 Associated X Protein (Bax) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.25ng/mL.
  • Intended UseHuman BAX ELISA Kit allows for the in vitro quantitative determination of BAX , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Associated X Protein (Bax). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bcl2 Associated X Protein (Bax). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Associated X Protein (Bax), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bcl2 Associated X Protein (Bax) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Apoptosis regulator BAX Protein information
  • Uniprot ID BAX_HUMAN
  • Uniprot AC Q9UCZ6; Q9UCZ7; Q9UQD6;
  • UniGene Hs.624291;
  • GeneID 581
  • KEGG hsa:581;
  • Human Apoptosis regulator BAX Protein SEQUENCE
  • SEQUENCE 192 AA; 21184 MW; 6C0CDB0A7DEE4994 CRC64;

    MDGSGEQPRG GGPTSSEQIM KTGALLLQGF IQDRAGRMGG EAPELALDPV

    PQDASTKKLS ECLKRIGDEL DSNMELQRMI AAVDTDSPRE VFFRVAADMF

    SDGNFNWGRV VALFYFASKL VLKALCTKVP ELIRTIMGWT LDFLRERLLG

    WIQDQGGWDG LLSYFGTPTW QTVTIFVAGV LTASLTIWKK MG

  • UCSC uc002plf.2; human. [Q07812-1];



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