Human TLR4 ELISA Kit

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  • Alternative name

    TLR4 ELISA KIT; TLR4 ELISA KIT; Toll-like receptor 4TLR4 ELISA KIT;

  • Catalog
    E038554
  • species
    Human
  • GeneTLR4
  • Standard CurveHuman TLR4 ELISA Kit
  • Other Species Mouse Tlr4 ELISA KitBovine TLR4 ELISA KitHamster TLR4 ELISA KitHorse TLR4 ELISA KitPorcine TLR4 ELISA KitRat Tlr4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of TLR-4. No significant cross-reactivity or interference between TLR-4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TLR-4 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman TLR4 ELISA Kit allows for the in vitro quantitative determination of TLR4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Intended Uses: This TLR-4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TLR-4. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||TLR-4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TLR-4. Standards or samples are then added to the microtiter plate wells and TLR-4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TLR-4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TLR-4 are added to each well to "sandwich" the TLR-4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TLR-4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TLR-4 concentration in each sample is interpolated from this standard curve.



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