Mouse Ctsd ELISA Kit

Alternative name
Mouse CD ELISA Kit;Mouse CatD ELISA Kit;Mouse cathepsin D ELISA Kit;
Catalog
E047414
species
Mouse
GeneCtsd
Standard Curve
Other Species Human CTSD ELISA Kit Bovine CTSD ELISA Kit Canine CTSD ELISA Kit Chicken CTSD ELISA Kit Porcine CTSD ELISA Kit Rat Ctsd ELISA Kit Sheep CTSD ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CTSD. No significant cross-reactivity or interference between CTSD and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CTSD and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseMouse Ctsd ELISA Kit allows for the in vitro quantitative determination of Ctsd , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalPrinciple of the assay: CTSD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CTSD antibody and an CTSD-HRP conjugate. The assay sample and buffer are incubated together with CTSD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CTSD concentration since CTSD from samples and CTSD-HRP conjugate compete for the anti-CTSD antibody binding site. Since the number of sites is limited, as more sites are occupied by CTSD from the sample, fewer sites are left to bind CTSD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTSD concentration in each sample is interpolated from this standard curve.
Mouse Cathepsin D Protein information
Uniprot ID CATD_MOUSE
Uniprot AC P18242;
UniGene Mm.231395;
GeneID 13033
KEGG mmu:13033;
Mouse Cathepsin D Protein SEQUENCE
SEQUENCE 410 AA; 44954 MW; DC4928EC46928BF0 CRC64;
MKTPGVLLLI LGLLASSSFA IIRIPLRKFT SIRRTMTEVG GSVEDLILKG
PITKYSMQSS PKTTEPVSEL LKNYLDAQYY GDIGIGTPPQ CFTVVFDTGS
SNLWVPSIHC KILDIACWVH HKYNSDKSST YVKNGTSFDI HYGSGSLSGY
LSQDTVSVPC KSDQSKARGI KVEKQIFGEA TKQPGIVFVA AKFDGILGMG
YPHISVNNVL PVFDNLMQQK LVDKNIFSFY LNRDPEGQPG GELMLGGTDS
KYYHGELSYL NVTRKAYWQV HMDQLEVGNE LTLCKGGCEA IVDTGTSLLV
GPVEEVKELQ KAIGAVPLIQ GEYMIPCEKV SSLPTVYLKL GGKNYELHPD
KYILKVSQGG KTICLSGFMG MDIPPPSGPL WILGDVFIGS YYTVFDRDNN
RVGFANAVVL
UCSC uc009kmv.1; mouse.;

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Mouse Ctsd ELISA Kit

Mouse CD ELISA Kit;Mouse CatD ELISA Kit;Mouse cathepsin D ELISA Kit;