Human ACTN2 ELISA Kit

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  • Alternative name

    Human Alpha-actinin skeletal muscle isoform 2 ELISA Kit;Human F-actin cross-linking protein ELISA Kit;Human CMD1AA ELISA Kit;Human CMH23 ELISA Kit;Human actinin alpha 2 ELISA Kit;Human alpha-actinin-2 ELISA Kit;Human alpha-actinin skeletal muscle ELISA Kit;

  • Catalog
    E000451
  • species
    Human
  • GeneACTN2
  • Standard CurveHuman ACTN2 ELISA Kit
  • Other Species Mouse Actn2 ELISA KitBovine ACTN2 ELISA KitChicken ACTN2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Actinin Alpha 2 (ACTN2). No significant cross-reactivity or interference between Actinin Alpha 2 (ACTN2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.122ng/mL.
  • Intended UseHuman ACTN2 ELISA Kit allows for the in vitro quantitative determination of ACTN2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actinin Alpha 2 (ACTN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Actinin Alpha 2 (ACTN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actinin Alpha 2 (ACTN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Actinin Alpha 2 (ACTN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Alpha-actinin-2 Protein information
  • Uniprot ID ACTN2_HUMAN
  • Uniprot AC P35609; B1ANE4; B2RCS5; Q86TF4; Q86TI8;
  • UniGene Hs.498178;
  • GeneID 88
  • KEGG hsa:88;
  • Human Alpha-actinin-2 Protein SEQUENCE
  • SEQUENCE 894 AA; 103854 MW; 7F612C1C3B3E2299 CRC64;

    MNQIEPGVQY NYVYDEDEYM IQEEEWDRDL LLDPAWEKQQ RKTFTAWCNS

    HLRKAGTQIE NIEEDFRNGL KLMLLLEVIS GERLPKPDRG KMRFHKIANV

    NKALDYIASK GVKLVSIGAE EIVDGNVKMT LGMIWTIILR FAIQDISVEE

    TSAKEGLLLW CQRKTAPYRN VNIQNFHTSW KDGLGLCALI HRHRPDLIDY

    SKLNKDDPIG NINLAMEIAE KHLDIPKMLD AEDIVNTPKP DERAIMTYVS

    CFYHAFAGAE QAETAANRIC KVLAVNQENE RLMEEYERLA SELLEWIRRT

    IPWLENRTPE KTMQAMQKKL EDFRDYRRKH KPPKVQEKCQ LEINFNTLQT

    KLRISNRPAF MPSEGKMVSD IAGAWQRLEQ AEKGYEEWLL NEIRRLERLE

    HLAEKFRQKA STHETWAYGK EQILLQKDYE SASLTEVRAL LRKHEAFESD

    LAAHQDRVEQ IAAIAQELNE LDYHDAVNVN DRCQKICDQW DRLGTLTQKR

    REALERMEKL LETIDQLHLE FAKRAAPFNN WMEGAMEDLQ DMFIVHSIEE

    IQSLITAHEQ FKATLPEADG ERQSIMAIQN EVEKVIQSYN IRISSSNPYS

    TVTMDELRTK WDKVKQLVPI RDQSLQEELA RQHANERLRR QFAAQANAIG

    PWIQNKMEEI ARSSIQITGA LEDQMNQLKQ YEHNIINYKN NIDKLEGDHQ

    LIQEALVFDN KHTNYTMEHI RVGWELLLTT IARTINEVET QILTRDAKGI

    TQEQMNEFRA SFNHFDRRKN GLMDHEDFRA CLISMGYDLG EAEFARIMTL

    VDPNGQGTVT FQSFIDFMTR ETADTDTAEQ VIASFRILAS DKPYILAEEL

    RRELPPDQAQ YCIKRMPAYS GPGSVPGALD YAAFSSALYG ESDL

  • UCSC uc001hyf.4; human. [P35609-1];


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