Human ACTN2 ELISA Kit

Alternative name
Human Alpha-actinin skeletal muscle isoform 2 ELISA Kit;Human F-actin cross-linking protein ELISA Kit;Human CMD1AA ELISA Kit;Human CMH23 ELISA Kit;Human actinin alpha 2 ELISA Kit;Human alpha-actinin-2 ELISA Kit;Human alpha-actinin skeletal muscle ELISA Kit;
Catalog
E000451
species
Human
GeneACTN2
Standard Curve
Other Species Mouse Actn2 ELISA Kit Bovine ACTN2 ELISA Kit Chicken ACTN2 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Actinin Alpha 2 (ACTN2). No significant cross-reactivity or interference between Actinin Alpha 2 (ACTN2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.122ng/mL.
Intended UseHuman ACTN2 ELISA Kit allows for the in vitro quantitative determination of ACTN2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actinin Alpha 2 (ACTN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Actinin Alpha 2 (ACTN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actinin Alpha 2 (ACTN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Actinin Alpha 2 (ACTN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Alpha-actinin-2 Protein information
Uniprot ID ACTN2_HUMAN
Uniprot AC P35609; B1ANE4; B2RCS5; Q86TF4; Q86TI8;
UniGene Hs.498178;
GeneID 88
KEGG hsa:88;
Human Alpha-actinin-2 Protein SEQUENCE
SEQUENCE 894 AA; 103854 MW; 7F612C1C3B3E2299 CRC64;
MNQIEPGVQY NYVYDEDEYM IQEEEWDRDL LLDPAWEKQQ RKTFTAWCNS
HLRKAGTQIE NIEEDFRNGL KLMLLLEVIS GERLPKPDRG KMRFHKIANV
NKALDYIASK GVKLVSIGAE EIVDGNVKMT LGMIWTIILR FAIQDISVEE
TSAKEGLLLW CQRKTAPYRN VNIQNFHTSW KDGLGLCALI HRHRPDLIDY
SKLNKDDPIG NINLAMEIAE KHLDIPKMLD AEDIVNTPKP DERAIMTYVS
CFYHAFAGAE QAETAANRIC KVLAVNQENE RLMEEYERLA SELLEWIRRT
IPWLENRTPE KTMQAMQKKL EDFRDYRRKH KPPKVQEKCQ LEINFNTLQT
KLRISNRPAF MPSEGKMVSD IAGAWQRLEQ AEKGYEEWLL NEIRRLERLE
HLAEKFRQKA STHETWAYGK EQILLQKDYE SASLTEVRAL LRKHEAFESD
LAAHQDRVEQ IAAIAQELNE LDYHDAVNVN DRCQKICDQW DRLGTLTQKR
REALERMEKL LETIDQLHLE FAKRAAPFNN WMEGAMEDLQ DMFIVHSIEE
IQSLITAHEQ FKATLPEADG ERQSIMAIQN EVEKVIQSYN IRISSSNPYS
TVTMDELRTK WDKVKQLVPI RDQSLQEELA RQHANERLRR QFAAQANAIG
PWIQNKMEEI ARSSIQITGA LEDQMNQLKQ YEHNIINYKN NIDKLEGDHQ
LIQEALVFDN KHTNYTMEHI RVGWELLLTT IARTINEVET QILTRDAKGI
TQEQMNEFRA SFNHFDRRKN GLMDHEDFRA CLISMGYDLG EAEFARIMTL
VDPNGQGTVT FQSFIDFMTR ETADTDTAEQ VIASFRILAS DKPYILAEEL
RRELPPDQAQ YCIKRMPAYS GPGSVPGALD YAAFSSALYG ESDL
UCSC uc001hyf.4; human. [P35609-1];

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Category

Human ACTN2 ELISA Kit

Human Alpha-actinin skeletal muscle isoform 2 ELISA Kit;Human F-actin cross-linking protein ELISA Kit;Human CMD1AA ELISA Kit;Human CMH23 ELISA Kit;Human actinin alpha 2 ELISA Kit;Human alpha-actinin-2 ELISA Kit;Human alpha-actinin skeletal muscle ELISA Kit;