Human AIRE ELISA Kit

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  • Alternative name

    Human autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein ELISA Kit;Human APECED protein ELISA Kit;Human APECED ELISA Kit;Human AIRE1 ELISA Kit;Human APS1 ELISA Kit;Human APSI ELISA Kit;Human PGA1 ELISA Kit;Human autoimmune regulator ELISA Kit;

  • Catalog
    E004246
  • species
    Human
  • GeneAIRE
  • Standard CurveHuman AIRE ELISA Kit
  • Other Species Mouse Aire ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Autoimmune Regulator (AIRE). No significant cross-reactivity or interference between Autoimmune Regulator (AIRE) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.053ng/mL.
  • Intended UseHuman AIRE ELISA Kit allows for the in vitro quantitative determination of AIRE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Autoimmune Regulator (AIRE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Autoimmune Regulator (AIRE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Autoimmune Regulator (AIRE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Autoimmune Regulator (AIRE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Autoimmune regulator Protein information
  • Uniprot ID AIRE_HUMAN
  • Uniprot AC O43918; B2RP50; O43922; O43932; O75745;
  • UniGene Hs.129829;
  • GeneID 326
  • KEGG hsa:326;
  • Human Autoimmune regulator Protein SEQUENCE
  • SEQUENCE 545 AA; 57727 MW; 8CF703F8C9411BC5 CRC64;

    MATDAALRRL LRLHRTEIAV AVDSAFPLLH ALADHDVVPE DKFQETLHLK

    EKEGCPQAFH ALLSWLLTQD STAILDFWRV LFKDYNLERY GRLQPILDSF

    PKDVDLSQPR KGRKPPAVPK ALVPPPRLPT KRKASEEARA AAPAALTPRG

    TASPGSQLKA KPPKKPESSA EQQRLPLGNG IQTMSASVQR AVAMSSGDVP

    GARGAVEGIL IQQVFESGGS KKCIQVGGEF YTPSKFEDSG SGKNKARSSS

    GPKPLVRAKG AQGAAPGGGE ARLGQQGSVP APLALPSDPQ LHQKNEDECA

    VCRDGGELIC CDGCPRAFHL ACLSPPLREI PSGTWRCSSC LQATVQEVQP

    RAEEPRPQEP PVETPLPPGL RSAGEEVRGP PGEPLAGMDT TLVYKHLPAP

    PSAAPLPGLD SSALHPLLCV GPEGQQNLAP GARCGVCGDG TDVLRCTHCA

    AAFHWRCHFP AGTSRPGTGL RCRSCSGDVT PAPVEGVLAP SPARLAPGPA

    KDDTASHEPA LHRDDLESLL SEHTFDGILQ WAIQSMARPA APFPS

  • UCSC uc002zei.4; human. [O43918-1];


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