Mouse Aqp5 ELISA Kit

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  • Alternative name

    Mouse AQP-5 ELISA Kit;Mouse aquaporin 5 ELISA Kit;Mouse aquaporin-5 ELISA Kit;

  • Catalog
    E044715
  • species
    Mouse
  • GeneAqp5
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of AQP5. No significant cross-reactivity or interference between AQP5 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.058ng/mL.
  • Intended UseMouse Aqp5 ELISA Kit allows for the in vitro quantitative determination of Aqp5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of AQP5 in mouse tissue homogenates, cell lysates and other biological fluids. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to AQP5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to AQP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AQP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of AQP5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Aquaporin-5 Protein information
  • Uniprot ID AQP5_MOUSE
  • Uniprot AC Q9WTY4; Q545H1;
  • UniGene Mm.45580;
  • GeneID 11830
  • KEGG mmu:11830;
  • Mouse Aquaporin-5 Protein SEQUENCE
  • SEQUENCE 265 AA; 28274 MW; 917DF4A0C0A4C620 CRC64;

    MKKEVCSVAF FKAVFAEFLA TLIFVFFGLG SALKWPSALP TILQISIAFG

    LAIGTLAQAL GPVSGGHINP AITLALLIGN QISLLRAIFY VAAQLVGAIA

    GAGILYWLAP GNARGNLAVN ALSNNTTPGK AVVVELILTF QLALCIFSST

    DSRRTSPVGS PALSIGLSVT LGHLVGIYFT GCSMNPARSF GPAVVMNRFS

    PSHWVFWVGP IVGAVLAAIL YFYLLFPSSL SLHDRVAVVK GTYEPEEDWE

    DHREERKKTI ELTAH

  • UCSC uc007xpt.1; mouse.;



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