Mouse Aars2 ELISA Kit

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  • Alternative name

    Mouse Alanyl-tRNA synthetase ELISA Kit;Mouse alaRS ELISA Kit;Mouse Aarsl ELISA Kit;Mouse Gm89 ELISA Kit;Mouse Kiaa1270 ELISA Kit;Mouse alanyl-tRNA synthetase 2, mitochondrial ELISA Kit;Mouse alanine--tRNA ligase, mitochondrial ELISA Kit;Mouse alanyl-tRNA synthetase 2, mitochondrial (putative) ELISA Kit;Mouse alanyl-tRNA synthetase like ELISA Kit;Mouse alanyl-tRNA synthetase, mitochondrial ELISA Kit;Mouse probable alanyl-tRNA synthetase, mitochondrial ELISA Kit;

  • Catalog
    E043143
  • species
    Mouse
  • GeneAars2
  • Standard CurveMouse Aars2 ELISA Kit
  • Other Species Human AARS2 ELISA KitRat Aars2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of AARS2. No significant cross-reactivity or interference between AARS2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AARS2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseMouse Aars2 ELISA Kit allows for the in vitro quantitative determination of Aars2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Principle of the assay: AARS2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AARS2 antibody and an AARS2-HRP conjugate. The assay sample and buffer are incubated together with AARS2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AARS2 concentration since AARS2 from samples and AARS2-HRP conjugate compete for the anti-AARS2 antibody binding site. Since the number of sites is limited, as more sites are occupied by AARS2 from the sample, fewer sites are left to bind AARS2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AARS2 concentration in each sample is interpolated from this standard curve.
  • Mouse Alanine--tRNA ligase, mitochondrial Protein information
  • Uniprot ID SYAM_MOUSE
  • Uniprot AC Q14CH7; Q68FH3; Q69ZM9;
  • UniGene Mm.329063;
  • GeneID 224805
  • KEGG mmu:224805;
  • Mouse Alanine--tRNA ligase, mitochondrial Protein SEQUENCE
  • SEQUENCE 980 AA; 106783 MW; 7734BC58CDB5463D CRC64;

    MAVALAAAAG KLRRAIGRSC PWQPFSTEPG PPHGAAVRDA FLSFFRDRHG

    HRLVPSATVR PRGDPSLLFV NAGMNQFKPI FLGTVDPRSE MAGFRRVVNS

    QKCVRAGGRH NDLEDVGRDL SHHTFFEMLG NWAFGGEYFK EEACSMAWEL

    LTQVYGIPED RLWVSYFSGD SQTGLDPDLE TRDIWLSLGV PASRVLSFGP

    QENFWEMGDT GPCGPCTEIH YDLAGGVGSP QLVELWNLVF MQHYREADGS

    LQLLPQRHVD TGMGLERLVA VLQGKRSTYD TDLFSPLLDA IHQSCGAPPY

    SGRVGAADEG RIDTAYRVVA DHIRTLSVCI ADGVSPGMSG APLVLRRILR

    RAVRYSTEVL QAPPGFLGSL VPVVVETLGS AYPELEKNSV KIASLVSEDE

    AAFLASLQRG RRIIDRTVKR LGPSDLFPAE VAWSLSLSGN LGIPLDLVEL

    MLEEKGVKLD TAGLEQLAQK EAQHRAQQAE ADQEDRLCLD VHALEELHRQ

    GIPTTDDSPK YNYTLHPNGD YEFGLCEARV LQLYSETGTA VASVGAGQRC

    GLLLDRTNFY AEQGGQASDR GYLVRTGQQD MLFPVAGAQL CGGFILHEAM

    APERLQVGDQ VQLYVDKAWR MGCMVKHTAT HLLSWALRQT LGPTTEQRGS

    HLNPERLRFD VATQTLLTTE QLRTVESYVQ EVVGQDKPVF MEEVPLAHTA

    RIPGLRSLDE VYPDPVRVVS VGVPVAHALG PASQAAMHTS VELCCGTHLL

    STGAVGDLVI IGERQLVKGI TRLLAITGEQ AQQAREVGQS LSQEVEAASE

    RLSQGSRDLP EAHRLSKDIG RLTEVAESAV IPQWQRQELQ TTLKMLQRRA

    NTAIRKLEKG QATEKSQELL KRHSEGPLIV DTVSAESLSV LVKVVRQLCK

    QAPSISVLLL SPQPTGSVLC ACQVAQDATP TFTAEAWALA VCSHMGGKAW

    GSRVVAQGTG HTADLEAALG TARAYALSQL

  • UCSC uc008cqr.1; mouse.;


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