SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse CCL12/MCP-5. No significant cross-reactivity or interference between Mouse CCL12/MCP-5 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CCL12/MCP-5 and all the analogues, therefore, cross reaction may still exist.
Intended UseMouse CCL12/MCP-5 ELISA Kit allows for the in vitro quantitative determination of CCL12/MCP-5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CCL12/MCP-5 in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CCL12/MCP-5. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CCL12/MCP-5 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CCL12/MCP-5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CCL12/MCP-5. You can calculate the concentration of Mouse CCL12/MCP-5 in the samples by comparing the OD of the samples to the standard curve.