Mouse Agxt ELISA Kit

Alternative name
Mouse SPT ELISA Kit;Mouse Alanine--glyoxylate aminotransferase ELISA Kit;Mouse AGT ELISA Kit;Mouse Agxt1 ELISA Kit;Mouse Agt1 ELISA Kit;Mouse alanine-glyoxylate aminotransferase ELISA Kit;Mouse serine--pyruvate aminotransferase, mitochondrial ELISA Kit;Mouse serine--pyruvate aminotransferase, peroxisomal ELISA Kit;Mouse alanine-glyoxylate aminotransferase 1 ELISA Kit;Mouse serine-pyruvate aminotransferase ELISA Kit;
Catalog
E043138
species
Mouse
GeneAgxt
SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse AGXT. No significant cross-reactivity or interference between mouse AGXT and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of mouse AGXT is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseMouse Agxt ELISA Kit allows for the in vitro quantitative determination of Agxt , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AGXT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AGXT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGXT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGXT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Serine--pyruvate aminotransferase, mitochondrial Protein information
Uniprot ID SPYA_MOUSE
Uniprot AC O35423; Q8R128;
UniGene Mm.7457;
GeneID 11611
KEGG mmu:11611;
Mouse Serine--pyruvate aminotransferase, mitochondrial Protein SEQUENCE
SEQUENCE 414 AA; 45912 MW; A2AB28AC48A292E3 CRC64;
MFRMLAKASV TLGSRAAGWV RTMGSYQLLV PPPEALSKPL SVPTRLLLGP
GPSNLAPRVL AAGSLRMIGH MQKEMLQIME EIKQGIQYVF QTRNPLTLVV
SGSGHCAMET ALFNLLEPGD SFLTGTNGIW GMRAAEIADR IGARVHQMIK
KPGEHYTLQE VEEGLAQHKP VLLFLVHGES STGVVQPLDG FGELCHRYQC
LLLVDSVASL GGVPIYMDQQ GIDIMYSSSQ KVLNAPPGIS LISFNDKAKY
KVYSRKTKPV SFYTDITYLA KLWGCEGETR VIHHTTPVTS LYCLRESLAL
IAEQGLENCW RRHREATAHL HKHLQEMGLK FFVKDPEIRL PTITTVTVPA
GYNWRDIVSY VLDHFSIEIS GGLGPTEERV LRIGLLGYNA TTENVDRVAE
ALREALQHCP KNKL
UCSC uc007cdi.2; mouse. [O35423-1];

Category

Mouse Agxt ELISA Kit