Mouse FKN ELISA Kit

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  • Alternative name

    Fractalkine ELISA KIT; C-X3-C motif chemokine 1 ELISA KIT; CX3C membrane-anchored chemokine ELISA KIT; Neurotactin ELISA KIT; Small-inducible cytokine D1CX3CL1 ELISA KIT; FKN ELISA KIT; NTT ELISA KIT; SCYD1 ELISA KIT

  • Catalog
    E049822
  • species
    Mouse
  • GeneFKN
  • Standard CurveMouse FKN ELISA Kit
  • Other Species Human FKN ELISA KitHuman FKN/CX3CL1 ELISA KitMouse FKN/CX3CL1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse FKN ELISA Kit allows for the in vitro quantitative determination of FKN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Principle of the Assay: FKN/CX3CL1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FKN/CX3CL1. Standards or samples are then added to the microtiter plate wells and FKN/CX3CL1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FKN/CX3CL1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for FKN/CX3CL1 are added to each well to "sandwich" the FKN/CX3CL1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FKN/CX3CL1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FKN/CX3CL1 concentration in each sample is interpolated from this standard curve.


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