Human RAET1G ELISA Kit

Alternative name
Human UL-16 binding protein 5 ELISA Kit;Human ULBP5 ELISA Kit;Human retinoic acid early transcript 1G ELISA Kit;Human retinoic acid early transcript 1G protein ELISA Kit;
Catalog
E033342
species
Human
GeneRAET1G
Standard Curve
Other Species Mouse RAET1G ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of RAET-1G. No significant cross-reactivity or interference between RAET-1G and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RAET-1G and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman RAET1G ELISA Kit allows for the in vitro quantitative determination of RAET1G , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: RAET-1G ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RAET-1G antibody and an RAET-1G-HRP conjugate. The assay sample and buffer are incubated together with RAET-1G-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RAET-1G concentration since RAET-1G from samples and RAET-1G-HRP conjugate compete for the anti-RAET-1G antibody binding site. Since the number of sites is limited, as more sites are occupied by RAET-1G from the sample, fewer sites are left to bind RAET-1G-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RAET-1G concentration in each sample is interpolated from this standard curve.
Human Retinoic acid early transcript 1G protein Protein information
Uniprot ID RET1G_HUMAN
Uniprot AC Q6H3X3; Q6H3X2;
UniGene Hs.535023; Hs.558792;
GeneID 353091
KEGG hsa:353091;
Human Retinoic acid early transcript 1G protein Protein SEQUENCE
SEQUENCE 334 AA; 37106 MW; 3F8350036951BA69 CRC64;
MAAAASPAFL LRLPLLLLLS SWCRTGLADP HSLCYDITVI PKFRPGPRWC
AVQGQVDEKT FLHYDCGSKT VTPVSPLGKK LNVTTAWKAQ NPVLREVVDI
LTEQLLDIQL ENYIPKEPLT LQARMSCEQK AEGHGSGSWQ LSFDGQIFLL
FDSENRMWTT VHPGARKMKE KWENDKDMTM SFHYISMGDC TGWLEDFLMG
MDSTLEPSAG APPTMSSGTA QPRATATTLI LCCLLIMCLL ICSRHSLTQS
HGHHPQSLQP PPHPPLLHPT WLLRRVLWSD SYQIAKRPLS GGHVTRVTLP
IIGDDSHSLP CPLALYTINN GAARYSEPLQ VSIS
UCSC uc010kii.1; human. [Q6H3X3-1];

Category

Human RAET1G ELISA Kit

Human UL-16 binding protein 5 ELISA Kit;Human ULBP5 ELISA Kit;Human retinoic acid early transcript 1G ELISA Kit;Human retinoic acid early transcript 1G protein ELISA Kit;