Mouse AGE ELISA Kit

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  • Catalog
    E043051
  • species
    Mouse
  • GeneAGE
  • Standard CurveMouse AGE ELISA Kit
  • Other Species Human AGE ELISA KitHuman CML-AGE ELISA KitHuman GTE-AGE ELISA KitHuman Hb AGE ELISA KitHuman HB-AGE ELISA KitMouse CML-AGE ELISA KitMouse Hb-AGE ELISA Kit
  • SpecificityThis assay recognizes recombinant and natural mouseAdvanced glycation end-product. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.39ng/ml.
  • Intended UseMouse AGE ELISA Kit allows for the in vitro quantitative determination of AGE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Intended Uses: This immunoassay kit allows for the in vitro quantitative determination ofmouseAdvanced glycation end-productconcentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to Advanced glycation end-product. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forAdvanced glycation end-product and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Advanced glycation end-product, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Advanced glycation end-product in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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