Mouse Il17a ELISA Kit

Alternative name
Mouse IL-17 ELISA Kit;Mouse IL-17A ELISA Kit;Mouse cytotoxic T-lymphocyte-associated antigen 8 ELISA Kit;Mouse Ctla-8 ELISA Kit;Mouse Ctla8 ELISA Kit;Mouse Il17 ELISA Kit;Mouse interleukin 17A ELISA Kit;Mouse interleukin-17A ELISA Kit;
Catalog
E056299
species
Mouse
GeneIl17a
SpecificityNatural and recombinant mouse IL-17
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 1pg/ml
Intended UseMouse Il17a ELISA Kit allows for the in vitro quantitative determination of Il17a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: mouse IL-17 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-17 has been precoated onto 96-well plates. Standards(E.coli,T22-A158) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-17 amount of sample captured in plate. Background: IL-17 is an inflammatory cytokine produced primarily by a unique lineage of CD4 T cells that plays critical roles in the pathogenesis of multiple autoimmune diseases. Interleukin-17 is expressed by activated T cells and is 57% identical to the 17- to 26-kD secretory glycoprotein encoded by gene 13 of the herpesvirus saimiri (HVS-13). IL17 induces nuclear factor kappa-B and the expression of IL6, intercellular adhesion molecule-1, granulocyte macrophage colony-stimulating factor,and prostaglandin E2, as well as the maturation of CD34 positive hematopoietic precursors into neutrophils. Anti-IL17 antibodies significantly inhibited osteoclast formation induced by culture media of RA synovial tissues. The standard product used in this kit is recombinant mouse IL-17, consisting of 137 amino acids with the molecular mass of 15.5KDa.
Mouse Interleukin-17A Protein information
Uniprot ID IL17_MOUSE
Uniprot AC Q62386; Q60971;
UniGene Mm.5419;
GeneID 16171
KEGG mmu:16171;
Mouse Interleukin-17A Protein SEQUENCE
SEQUENCE 158 AA; 17490 MW; 3505C143435F4653 CRC64;
MSPGRASSVS LMLLLLLSLA ATVKAAAIIP QSSACPNTEA KDFLQNVKVN
LKVFNSLGAK VSSRRPSDYL NRSTSPWTLH RNEDPDRYPS VIWEAQCRHQ
RCVNAEGKLD HHMNSVLIQQ EILVLKREPE SCPFTFRVEK MLVGVGCTCV
ASIVRQAA
UCSC uc007aky.1; mouse.;

Mouse Il17a ELISA Kit

Mouse IL-17 ELISA Kit;Mouse IL-17A ELISA Kit;Mouse cytotoxic T-lymphocyte-associated antigen 8 ELISA Kit;Mouse Ctla-8 ELISA Kit;Mouse Ctla8 ELISA Kit;Mouse Il17 ELISA Kit;Mouse interleukin 17A ELISA Kit;Mouse interleukin-17A ELISA Kit;

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