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Mouse Ak2 ELISA Kit

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  • Alternative name

    Mouse AK 2 ELISA Kit;Mouse ATP-AMP transphosphorylase 2 ELISA Kit;Mouse ATP:AMP phosphotransferase ELISA Kit;Mouse adenylate monophosphate kinase ELISA Kit;Mouse Ak-2 ELISA Kit;Mouse D4Ertd220e ELISA Kit;Mouse adenylate kinase 2 ELISA Kit;Mouse adenylate kinase 2, mitochondrial ELISA Kit;
  • Catalog
    E042881
  • species
    Mouse
  • GeneAk2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of AK2. No significant cross-reactivity or interference between AK2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AK2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Ak2 ELISA Kit allows for the in vitro quantitative determination of Ak2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: AK2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AK2 antibody and an AK2-HRP conjugate. The assay sample and buffer are incubated together with AK2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AK2 concentration since AK2 from samples and AK2-HRP conjugate compete for the anti-AK2 antibody binding site. Since the number of sites is limited, as more sites are occupied by AK2 from the sample, fewer sites are left to bind AK2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AK2 concentration in each sample is interpolated from this standard curve.
  • Mouse Adenylate kinase 2, mitochondrial, N-terminally processed Protein information
  • Uniprot ID KAD2_MOUSE
  • Uniprot AC Q9WTP6; A2A820; Q3THT3; Q3TI11; Q3TKI6; Q8C7I9; Q9CY37;
  • UniGene Mm.29460;
  • GeneID 11637
  • KEGG mmu:11637;
  • Mouse Adenylate kinase 2, mitochondrial, N-terminally processed Protein SEQUENCE

  • SEQUENCE 239 AA; 26469 MW; CAFF06F053CECE36 CRC64;

    MAPNVLASEP EIPKGIRAVL LGPPGAGKGT QAPKLAENFC VCHLATGDML

    RAMVASGSEL GKKLKATMDA GKLVSDEMVV ELIEKNLETP SCKNGFLLDG

    FPRTVRQAEM LDDLMEKRKE KLDSVIEFSI QDSLLIRRIT GRLIHPKSGR

    SYHEEFNPPK EPMKDDITGE PLIRRSDDNE KALKTRLEAY HTQTTPLVEY

    YRKRGIHCAI DASQTPDIVF ASILAAFSKA TCKDLVMFI

  • UCSC uc008uvu.2; mouse. [Q9WTP6-2];



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