Human ATRN ELISA Kit

Alternative name
Human DPPT-L ELISA Kit;Human mahogany homolog ELISA Kit;Human KIAA0548 ELISA Kit;Human MGCA ELISA Kit;Human attractin ELISA Kit;Human attractin-2 ELISA Kit;Human mahogany protein ELISA Kit;
Catalog
E004225
species
Human
GeneATRN
Standard Curve
Other Species Mouse Atrn ELISA Kit Rat Atrn ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman ATRN ELISA Kit allows for the in vitro quantitative determination of ATRN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalPrinciple of the Assay: ATRN ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ATRN. Standards or samples are then added to the microtiter plate wells and ATRN if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ATRN present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for ATRN are added to each well to "sandwich" the ATRN immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ATRN and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATRN concentration in each sample is interpolated from this standard curve.
Human Attractin Protein information
Uniprot ID ATRN_HUMAN
Uniprot AC Q9NTQ3; Q9NTQ4; Q9NU01; Q9NZ57; Q9NZ58; Q9UC75; Q9UDF5;
UniGene Hs.276252;
GeneID 8455
KEGG hsa:8455;
Human Attractin Protein SEQUENCE
SEQUENCE 1429 AA; 158537 MW; 9F206A319B7E3DD8 CRC64;
MVAAAAATEA RLRRRTAATA ALAGRSGGPH WDWDVTRAGR PGLGAGLRLP
RLLSPPLRPR LLLLLLLLSP PLLLLLLPCE AEAAAAAAAV SGSAAAEAKE
CDRPCVNGGR CNPGTGQCVC PAGWVGEQCQ HCGGRFRLTG SSGFVTDGPG
NYKYKTKCTW LIEGQPNRIM RLRFNHFATE CSWDHLYVYD GDSIYAPLVA
AFSGLIVPER DGNETVPEVV ATSGYALLHF FSDAAYNLTG FNITYSFDMC
PNNCSGRGEC KISNSSDTVE CECSENWKGE ACDIPHCTDN CGFPHRGICN
SSDVRGCSCF SDWQGPGCSV PVPANQSFWT REEYSNLKLP RASHKAVVNG
NIMWVVGGYM FNHSDYNMVL AYDLASREWL PLNRSVNNVV VRYGHSLALY
KDKIYMYGGK IDSTGNVTNE LRVFHIHNES WVLLTPKAKE QYAVVGHSAH
IVTLKNGRVV MLVIFGHCPL YGYISNVQEY DLDKNTWSIL HTQGALVQGG
YGHSSVYDHR TRALYVHGGY KAFSANKYRL ADDLYRYDVD TQMWTILKDS
RFFRYLHTAV IVSGTMLVFG GNTHNDTSMS HGAKCFSSDF MAYDIACDRW
SVLPRPDLHH DVNRFGHSAV LHNSTMYVFG GFNSLLLSDI LVFTSEQCDA
HRSEAACLAA GPGIRCVWNT GSSQCISWAL ATDEQEEKLK SECFSKRTLD
HDRCDQHTDC YSCTANTNDC HWCNDHCVPR NHSCSEGQIS IFRYENCPKD
NPMYYCNKKT SCRSCALDQN CQWEPRNQEC IALPENICGI GWHLVGNSCL
KITTAKENYD NAKLFCRNHN ALLASLTTQK KVEFVLKQLR IMQSSQSMSK
LTLTPWVGLR KINVSYWCWE DMSPFTNSLL QWMPSEPSDA GFCGILSEPS
TRGLKAATCI NPLNGSVCER PANHSAKQCR TPCALRTACG DCTSGSSECM
WCSNMKQCVD SNAYVASFPF GQCMEWYTMS TCPPENCSGY CTCSHCLEQP
GCGWCTDPSN TGKGKCIEGS YKGPVKMPSQ APTGNFYPQP LLNSSMCLED
SRYNWSFIHC PACQCNGHSK CINQSICEKC ENLTTGKHCE TCISGFYGDP
TNGGKCQPCK CNGHASLCNT NTGKCFCTTK GVKGDECQLC EVENRYQGNP
LRGTCYYTLL IDYQFTFSLS QEDDRYYTAI NFVATPDEQN RDLDMFINAS
KNFNLNITWA ASFSAGTQAG EEMPVVSKTN IKEYKDSFSN EKFDFRNHPN
ITFFVYVSNF TWPIKIQIAF SQHSNFMDLV QFFVTFFSCF LSLLLVAAVV
WKIKQSCWAS RRREQLLREM QQMASRPFAS VNVALETDEE PPDLIGGSIK
TVPKPIALEP CFGNKAAVLS VFVRLPRGLG GIPPPGQSGL AVASALVDIS
QQMPIVYKEK SGAVRNRKQQ PPAQPGTCI
UCSC uc002wil.3; human. [O75882-1];

Category

Human ATRN ELISA Kit

Human DPPT-L ELISA Kit;Human mahogany homolog ELISA Kit;Human KIAA0548 ELISA Kit;Human MGCA ELISA Kit;Human attractin ELISA Kit;Human attractin-2 ELISA Kit;Human mahogany protein ELISA Kit;