Mouse Adra1b ELISA Kit

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  • Alternative name

    Mouse Alpha-1B adrenoreceptor ELISA Kit;Mouse Alpha-1B adrenoceptor ELISA Kit;

  • Catalog
    E043341
  • species
    Mouse
  • GeneAdra1b
  • Standard CurveMouse Adra1b ELISA Kit
  • Other Species Human ADRA1B ELISA KitCanine ADRA1B ELISA KitRat Adra1b ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ADRA1B. No significant cross-reactivity or interference between ADRA1B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADRA1B and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Adra1b ELISA Kit allows for the in vitro quantitative determination of Adra1b , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This ADRA1B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ADRA1B. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ADRA1B ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ADRA1B antibody and an ADRA1B-HRP conjugate. The assay sample and buffer are incubated together with ADRA1B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADRA1B concentration since ADRA1B from samples and ADRA1B-HRP conjugate compete for the anti-ADRA1B antibody binding site. Since the number of sites is limited, as more sites are occupied by ADRA1B from the sample, fewer sites are left to bind ADRA1B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADRA1B concentration in each sample is interpolated from this standard curve.
  • Mouse Alpha-1B adrenergic receptor Protein information
  • Uniprot ID ADA1B_MOUSE
  • Uniprot AC P97717;
  • UniGene Mm.39086; Mm.487939;
  • Mouse Alpha-1B adrenergic receptor Protein SEQUENCE
  • SEQUENCE 514 AA; 56418 MW; 17CFD10123DC2B29 CRC64;

    MNPDLDTGHN TSAPAHWGEL KDANFTGPNQ TSSNSTLPQL DVTRAISVGC

    LGAFILFAIV GNILVILSVA CNRHLRTPTN YFIVNLAIAD LLLSFTDLPF

    SATLEVLGYW VLGRIFCDIW AAVDVLCCTA SILSLCAISI DRYIGVRYSL

    QYPTLVTRRK AILALLSVWV LSTVISIGPL LGWKEPAPND DKECGVTEEP

    FYALFSSLGS FYIPLAVILV MYCRVYIVAK RTTKNLEAGV MKEMSNSKEL

    TLRIHSKNFH EDTLSSTKAK GHNPRSSIAV KLFKFSREKK AAKTLGIVVG

    MFILCWLPFF IALPLGSLFS TLKPPDAVFK VVFWLGYFNS CLNPIIYPCS

    SKEFKRAFMR ILGCQCRGGR RRRRRRRLGA CAYTYRPWTR GGSLERSQSR

    KDSLDDSGSC MSGSQRTLPS ASPSPGYLGR GTQPPVELCA FPEWKPGALL

    SLPEPPGRRG RLDSGPLFTF KLLGEPESPG TEGDASNGGC DTTTDLANGQ

    PGFKSNMPLA PGHF



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