Human HTR1A ELISA Kit

Alternative name
Human 5-HT-1A ELISA Kit;Human 5-HT1A ELISA Kit;Human G-21 ELISA Kit;Human Serotonin receptor 1A ELISA Kit;Human ADRB2RL1 ELISA Kit;Human ADRBRL1 ELISA Kit;Human 5HT1a ELISA Kit;Human PFMCD ELISA Kit;Human 5-hydroxytryptamine receptor 1A ELISA Kit;Human 5-HT1a receptor ELISA Kit;Human 5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled ELISA Kit;Human guanine nucleotide-binding regulatory protein-coupled receptor ELISA Kit;
Catalog
E042066
species
Human
GeneHTR1A
SpecificityThis assay has high sensitivity and excellent specificity for detection of human HTR1A. No significant cross-reactivity or interference between human HTR1A and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human HTR1A is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman HTR1A ELISA Kit allows for the in vitro quantitative determination of HTR1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HTR1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HTR1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HTR1A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HTR1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human 5-hydroxytryptamine receptor 1A Protein information
Uniprot ID 5HT1A_HUMAN
Uniprot AC P08908; Q6LAE7;
UniGene Hs.247940;
GeneID 3350
KEGG hsa:3350;
Human 5-hydroxytryptamine receptor 1A Protein SEQUENCE
SEQUENCE 422 AA; 46107 MW; 762664FCF62CFD8F CRC64;
MDVLSPGQGN NTTSPPAPFE TGGNTTGISD VTVSYQVITS LLLGTLIFCA
VLGNACVVAA IALERSLQNV ANYLIGSLAV TDLMVSVLVL PMAALYQVLN
KWTLGQVTCD LFIALDVLCC TSSILHLCAI ALDRYWAITD PIDYVNKRTP
RRAAALISLT WLIGFLISIP PMLGWRTPED RSDPDACTIS KDHGYTIYST
FGAFYIPLLL MLVLYGRIFR AARFRIRKTV KKVEKTGADT RHGASPAPQP
KKSVNGESGS RNWRLGVESK AGGALCANGA VRQGDDGAAL EVIEVHRVGN
SKEHLPLPSE AGPTPCAPAS FERKNERNAE AKRKMALARE RKTVKTLGII
MGTFILCWLP FFIVALVLPF CESSCHMPTL LGAIINWLGY SNSLLNPVIY
AYFNKDFQNA FKKIIKCKFC RQ

Category

Human HTR1A ELISA Kit