Human FN3K ELISA Kit

Alternative name
Human fructosamine 3 kinase ELISA Kit;Human fructosamine-3-kinase ELISA Kit;
Catalog
E015603
species
Human
GeneFN3K
SpecificityThis assay has high sensitivity and excellent specificity for detection of Fructosamine-3-Kinase (FN3K). No significant cross-reactivity or interference between Fructosamine-3-Kinase (FN3K) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.122ng/mL.
Intended UseHuman FN3K ELISA Kit allows for the in vitro quantitative determination of FN3K , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fructosamine-3-Kinase (FN3K). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fructosamine-3-Kinase (FN3K). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fructosamine-3-Kinase (FN3K), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fructosamine-3-Kinase (FN3K) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Fructosamine-3-kinase Protein information
Uniprot ID FN3K_HUMAN
Uniprot AC Q9H479;
UniGene Hs.151135;
GeneID 64122
KEGG hsa:64122;
Human Fructosamine-3-kinase Protein SEQUENCE
SEQUENCE 309 AA; 35171 MW; BA886A86655DE28F CRC64;
MEQLLRAELR TATLRAFGGP GAGCISEGRA YDTDAGPVFV KVNRRTQARQ
MFEGEVASLE ALRSTGLVRV PRPMKVIDLP GGGAAFVMEH LKMKSLSSQA
SKLGEQMADL HLYNQKLREK LKEEENTVGR RGEGAEPQYV DKFGFHTVTC
CGFIPQVNEW QDDWPTFFAR HRLQAQLDLI EKDYADREAR ELWSRLQVKI
PDLFCGLEIV PALLHGDLWS GNVAEDDVGP IIYDPASFYG HSEFELAIAL
MFGGFPRSFF TAYHRKIPKA PGFDQRLLLY QLFNYLNHWN HFGREYRSPS
LGTMRRLLK
UCSC uc010wvs.2; human.;

Category

Human FN3K ELISA Kit

Human fructosamine 3 kinase ELISA Kit;Human fructosamine-3-kinase ELISA Kit;