Human FN3K ELISA Kit

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  • Alternative name

    Human fructosamine 3 kinase ELISA Kit;Human fructosamine-3-kinase ELISA Kit;

  • Catalog
    E015603
  • species
    Human
  • GeneFN3K
  • Standard CurveHuman FN3K ELISA Kit
  • Other Species Mouse Fn3k ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Fructosamine-3-Kinase (FN3K). No significant cross-reactivity or interference between Fructosamine-3-Kinase (FN3K) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.122ng/mL.
  • Intended UseHuman FN3K ELISA Kit allows for the in vitro quantitative determination of FN3K , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fructosamine-3-Kinase (FN3K). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fructosamine-3-Kinase (FN3K). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fructosamine-3-Kinase (FN3K), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fructosamine-3-Kinase (FN3K) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Fructosamine-3-kinase Protein information
  • Uniprot ID FN3K_HUMAN
  • Uniprot AC Q9H479;
  • UniGene Hs.151135;
  • GeneID 64122
  • KEGG hsa:64122;
  • Human Fructosamine-3-kinase Protein SEQUENCE
  • SEQUENCE 309 AA; 35171 MW; BA886A86655DE28F CRC64;

    MEQLLRAELR TATLRAFGGP GAGCISEGRA YDTDAGPVFV KVNRRTQARQ

    MFEGEVASLE ALRSTGLVRV PRPMKVIDLP GGGAAFVMEH LKMKSLSSQA

    SKLGEQMADL HLYNQKLREK LKEEENTVGR RGEGAEPQYV DKFGFHTVTC

    CGFIPQVNEW QDDWPTFFAR HRLQAQLDLI EKDYADREAR ELWSRLQVKI

    PDLFCGLEIV PALLHGDLWS GNVAEDDVGP IIYDPASFYG HSEFELAIAL

    MFGGFPRSFF TAYHRKIPKA PGFDQRLLLY QLFNYLNHWN HFGREYRSPS

    LGTMRRLLK

  • UCSC uc010wvs.2; human.;


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