Human HMGCR ELISA Kit

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  • Alternative name

    Human HMG-CoA reductase ELISA Kit;Human LDLCQ3 ELISA Kit;Human 3-hydroxy-3-methylglutaryl-CoA reductase ELISA Kit;Human 3-hydroxy-3-methylglutaryl-Coenzyme A reductase ELISA Kit;Human 3-hydroxy-3-methylglutaryl CoA reductase (NADPH) ELISA Kit;Human hydroxymethylglutaryl-CoA reductase ELISA Kit;

  • Catalog
    E041913
  • species
    Human
  • GeneHMGCR
  • Standard CurveHuman HMGCR ELISA Kit
  • Other Species Human HMGCR/HMG-CoAR ELISA KitMouse Hmgcr ELISA KitMouse HMGCR/HMG-CoAR ELISA KitBovine HMGCR ELISA KitHamster HMGCR ELISA KitPorcine HMGCR ELISA KitRabbit HMGCR ELISA KitRat Hmgcr ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR). No significant cross-reactivity or interference between 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.58ng/mL.
  • Intended UseHuman HMGCR ELISA Kit allows for the in vitro quantitative determination of HMGCR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human 3-hydroxy-3-methylglutaryl-coenzyme A reductase Protein information
  • Uniprot ID HMDH_HUMAN
  • Uniprot AC P04035; B7Z3Y9; Q8N190;
  • UniGene Hs.628096; Hs.643495;
  • GeneID 3156
  • KEGG hsa:3156;
  • Human 3-hydroxy-3-methylglutaryl-coenzyme A reductase Protein SEQUENCE
  • SEQUENCE 888 AA; 97476 MW; 49B610DCCCFA26B6 CRC64;

    MLSRLFRMHG LFVASHPWEV IVGTVTLTIC MMSMNMFTGN NKICGWNYEC

    PKFEEDVLSS DIIILTITRC IAILYIYFQF QNLRQLGSKY ILGIAGLFTI

    FSSFVFSTVV IHFLDKELTG LNEALPFFLL LIDLSRASTL AKFALSSNSQ

    DEVRENIARG MAILGPTFTL DALVECLVIG VGTMSGVRQL EIMCCFGCMS

    VLANYFVFMT FFPACVSLVL ELSRESREGR PIWQLSHFAR VLEEEENKPN

    PVTQRVKMIM SLGLVLVHAH SRWIADPSPQ NSTADTSKVS LGLDENVSKR

    IEPSVSLWQF YLSKMISMDI EQVITLSLAL LLAVKYIFFE QTETESTLSL

    KNPITSPVVT QKKVPDNCCR REPMLVRNNQ KCDSVEEETG INRERKVEVI

    KPLVAETDTP NRATFVVGNS SLLDTSSVLV TQEPEIELPR EPRPNEECLQ

    ILGNAEKGAK FLSDAEIIQL VNAKHIPAYK LETLMETHER GVSIRRQLLS

    KKLSEPSSLQ YLPYRDYNYS LVMGACCENV IGYMPIPVGV AGPLCLDEKE

    FQVPMATTEG CLVASTNRGC RAIGLGGGAS SRVLADGMTR GPVVRLPRAC

    DSAEVKAWLE TSEGFAVIKE AFDSTSRFAR LQKLHTSIAG RNLYIRFQSR

    SGDAMGMNMI SKGTEKALSK LHEYFPEMQI LAVSGNYCTD KKPAAINWIE

    GRGKSVVCEA VIPAKVVREV LKTTTEAMIE VNINKNLVGS AMAGSIGGYN

    AHAANIVTAI YIACGQDAAQ NVGSSNCITL MEASGPTNED LYISCTMPSI

    EIGTVGGGTN LLPQQACLQM LGVQGACKDN PGENARQLAR IVCGTVMAGE

    LSLMAALAAG HLVKSHMIHN RSKINLQDLQ GACTKKTA

  • UCSC uc003kdp.4; human. [P04035-1];



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