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Alternative name
Natriuretic peptides B ELISA KIT; Gamma-brain natriuretic peptideNPPB ELISA KIT; BNP(1-32) ELISA KIT; BNP-32 ELISA KIT
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Catalog
E005235
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species
Human
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GeneBNP
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Standard Curve
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Other Species
Human NT BNP ELISA KitMouse BNP ELISA KitMouse NT-BNP ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Brain Natriuretic Peptide (BNP).
No significant cross-reactivity or interference between Brain Natriuretic Peptide (BNP) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 9.24pg/mL.
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Intended UseHuman BNP ELISA Kit allows for the in vitro quantitative determination of BNP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Brain Natriuretic Peptide (BNP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Brain Natriuretic Peptide (BNP) and unlabeled Brain Natriuretic Peptide (BNP) (Standards or samples) with the pre-coated antibody specific to Brain Natriuretic Peptide (BNP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Brain Natriuretic Peptide (BNP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Brain Natriuretic Peptide (BNP) in the sample.
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