SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse CD247. No significant cross-reactivity or interference between mouse CD247 and analogues was observed.
Intended UseMouse Cd247 ELISA Kit allows for the in vitro quantitative determination of Cd247 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD247 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD247 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD247 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD247 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse T-cell surface glycoprotein CD3 zeta chain Protein information
Uniprot ID
CD3Z_MOUSE
Uniprot AC
P24161; P29020; Q9D3G3;
UniGene
Mm.217308; Mm.245261; Mm.475205;
GeneID
12503
KEGG
mmu:12503;
Mouse T-cell surface glycoprotein CD3 zeta chain Protein SEQUENCE