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Human TPH1 ELISA Kit

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  • Alternative name

    Human tryptophan 5-monooxygenase 1 ELISA Kit;Human TPH ELISA Kit;Human TPRH ELISA Kit;Human TRPH ELISA Kit;Human tryptophan hydroxylase 1 ELISA Kit;Human tryptophan 5-hydroxylase 1 ELISA Kit;Human L-tryptophan hydroxylase ELISA Kit;Human indoleacetic acid-5-hydroxylase ELISA Kit;Human tryptophan hydroxylase (tryptophan 5-monooxygenase) ELISA Kit;
  • Catalog
    E038690
  • species
    Human
  • GeneTPH1
  • Other Species Mouse Tph1 ELISA KitChicken TPH1 ELISA KitRabbit TPH1 ELISA KitRat Tph1 ELISA Kit
  • SpecificityThis kit recognizes natural and recombinant Human TPH1. No significant cross-reactivity or interference between Human TPH1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of Human TPH1 is 0.188ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
  • Intended UseHuman TPH1 ELISA Kit allows for the in vitro quantitative determination of TPH1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TPH1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TPH1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TPH1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TPH1. You can calculate the concentration of TPH1 in the samples by comparing the OD of the samples to the standard curve.
  • Human Tryptophan 5-hydroxylase 1 Protein information
  • Uniprot ID TPH1_HUMAN
  • Uniprot AC P17752; D3DQX6; O95188; O95189; Q16736; Q3KPG8;
  • UniGene Hs.591999;
  • GeneID 7166
  • KEGG hsa:7166;
  • Human Tryptophan 5-hydroxylase 1 Protein SEQUENCE

  • SEQUENCE 444 AA; 50985 MW; DFAD501446953A91 CRC64;

    MIEDNKENKD HSLERGRASL IFSLKNEVGG LIKALKIFQE KHVNLLHIES

    RKSKRRNSEF EIFVDCDINR EQLNDIFHLL KSHTNVLSVN LPDNFTLKED

    GMETVPWFPK KISDLDHCAN RVLMYGSELD ADHPGFKDNV YRKRRKYFAD

    LAMNYKHGDP IPKVEFTEEE IKTWGTVFQE LNKLYPTHAC REYLKNLPLL

    SKYCGYREDN IPQLEDVSNF LKERTGFSIR PVAGYLSPRD FLSGLAFRVF

    HCTQYVRHSS DPFYTPEPDT CHELLGHVPL LAEPSFAQFS QEIGLASLGA

    SEEAVQKLAT CYFFTVEFGL CKQDGQLRVF GAGLLSSISE LKHALSGHAK

    VKPFDPKITC KQECLITTFQ DVYFVSESFE DAKEKMREFT KTIKRPFGVK

    YNPYTRSIQI LKDTKSITSA MNELQHDLDV VSDALAKVSR KPSI

  • UCSC uc001mnp.3; human. [P17752-1];


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