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Human NFKBID ELISA Kit

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  • Alternative name

    Human I-kappa-B-delta ELISA Kit;Human ikB-delta ELISA Kit;Human ikappaBdelta ELISA Kit;Human IkappaBNS ELISA Kit;Human T-cell activation NFKB-like protein ELISA Kit;Human TA-NFKBH ELISA Kit;Human IKBNS ELISA Kit;Human NFKB inhibitor delta ELISA Kit;Human NF-kappa-B inhibitor delta ELISA Kit;Human nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, delta ELISA Kit;
  • Catalog
    E026461
  • species
    Human
  • GeneNFKBID
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of N1-N12. No significant cross-reactivity or interference between N1-N12 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between N1-N12 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman NFKBID ELISA Kit allows for the in vitro quantitative determination of NFKBID , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: N1-N12 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-N1-N12 antibody and an N1-N12-HRP conjugate. The assay sample and buffer are incubated together with N1-N12-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the N1-N12 concentration since N1-N12 from samples and N1-N12-HRP conjugate compete for the anti-N1-N12 antibody binding site. Since the number of sites is limited, as more sites are occupied by N1-N12 from the sample, fewer sites are left to bind N1-N12-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The N1-N12 concentration in each sample is interpolated from this standard curve.
  • Human NF-kappa-B inhibitor delta Protein information
  • Uniprot ID IKBD_HUMAN
  • Uniprot AC Q8NI38; Q8NI39; Q9BRG9;
  • UniGene Hs.466531;
  • GeneID 84807
  • KEGG hsa:84807;
  • Human NF-kappa-B inhibitor delta Protein SEQUENCE

  • SEQUENCE 313 AA; 33481 MW; B986234830428320 CRC64;

    MEAGPWRVSA PPSGPPQFPA VVPGPSLEVA RAHMLALGPQ QLLAQDEEGD

    TLLHLFAARG LRWAAYAAAE VLQVYRRLDI REHKGKTPLL VAAAANQPLI

    VEDLLNLGAE PNAADHQGRS VLHVAATYGL PGVLLAVLNS GVQVDLEARD

    FEGLTPLHTA ILALNVAMRP SDLCPRVLST QARDRLDCVH MLLQMGANHT

    SQEIKSNKTV LHLAVQAANP TLVQLLLELP RGDLRTFVNM KAHGNTALHM

    AAALPPGPAQ EAIVRHLLAA GADPTLRNLE NEQPVHLLRP GPGPEGLRQL

    LKRSRVAPPG LSS

  • UCSC uc002oci.1; human. [Q8NI38-1];



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