Human GTF2A1 ELISA Kit

Alternative name
Human general transcription factor IIA subunit 1 ELISA Kit;Human TFIIAL ELISA Kit;Human transcription initiation factor TFIIA 42 kDa subunit ELISA Kit;Human TFIIA-42 ELISA Kit;Human TFIIA p35 subunit ELISA Kit;Human TFIIA p19 subunit ELISA Kit;Human TF2A1 ELISA Kit;Human TFIIA ELISA Kit;Human transcription initiation factor IIA subunit 1 ELISA Kit;Human TFIIA alpha, p55 ELISA Kit;Human TFIIA alpha/beta subunits ELISA Kit;Human general transcription factor IIA 1 ELISA Kit;Human general transcription factor IIA, 1, 19/37kDa ELISA Kit;Human glucose regulated protein, 58kD pseudogene ELISA Kit;
Catalog
E017678
species
Human
GeneGTF2A1
Other Species Mouse Gtf2a1 ELISA Kit Rat Gtf2a1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human GTF2A1. No significant cross-reactivity or interference between Human GTF2A1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human GTF2A1 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.188ng/mL
Intended UseHuman GTF2A1 ELISA Kit allows for the in vitro quantitative determination of GTF2A1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GTF2A1 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GTF2A1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human GTF2A1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GTF2A1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human GTF2A1. You can calculate the concentration of Human GTF2A1 in the samples by comparing the OD of the samples to the standard curve.
Human Transcription initiation factor IIA beta chain Protein information
Uniprot ID TF2AA_HUMAN
Uniprot AC P52655; Q3KNQ9;
UniGene Hs.592334;
GeneID 2957
KEGG hsa:2957;
Human Transcription initiation factor IIA beta chain Protein SEQUENCE
SEQUENCE 376 AA; 41514 MW; 4BAE1853E7E89218 CRC64;
MANSANTNTV PKLYRSVIED VINDVRDIFL DDGVDEQVLM ELKTLWENKL
MQSRAVDGFH SEEQQLLLQV QQQHQPQQQQ HHHHHHHQQA QPQQTVPQQA
QTQQVLIPAS QQATAPQVIV PDSKLIQHMN ASNMSAAATA ATLALPAGVT
PVQQILTNSG QLLQVVRAAN GAQYIFQPQQ SVVLQQQVIP QMQPGGVQAP
VIQQVLAPLP GGISPQTGVI IQPQQILFTG NKTQVIPTTV AAPTPAQAQI
TATGQQQPQA QPAQTQAPLV LQVDGTGDTS SEEDEDEEED YDDDEEEDKE
KDGAEDGQVE EEPLNSEDDV SDEEGQELFD TENVVVCQYD KIHRSKNKWK
FHLKDGIMNL NGRDYIFSKA IGDAEW
UCSC uc001xvf.4; human. [P52655-1];

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Human GTF2A1 ELISA Kit