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Alternative name
Interleukin-1 receptor antagonist protein ELISA KIT; ICIL-1RA ELISA KIT; IL1 inhibitor ELISA KIT; INN: AnakinraIL1RN ELISA KIT; IL1F3 ELISA KIT; IL1RA ELISA KIT; IL-1RN ELISA KIT; IL-1ra ELISA KIT; IRAP ELISA KIT
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Catalog
E057135
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species
Mouse
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GeneIL-1RA
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Standard Curve
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Other Species
Human IL-1ra/IL-1F3 ELISA KitHuman IL-1ra ELISA KitHuman IL-1ra/CD121 ELISA KitMouse IL-1ra/CD121 ELISA Kit
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SpecificityNo cross-reaction with other factors.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.05 ng/ml
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Intended UseMouse IL-1RA ELISA Kit allows for the in vitro quantitative determination of IL-1RA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse IL-1RA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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