Human TLR7 ELISA Kit

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  • Alternative name

    Human TLR7-like ELISA Kit;Human toll like receptor 7 ELISA Kit;Human toll-like receptor 7 ELISA Kit;Human toll-like receptor 7-like ELISA Kit;

  • Catalog
    E038541
  • species
    Human
  • GeneTLR7
  • Standard CurveHuman TLR7 ELISA Kit
  • Other Species Mouse Tlr7 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman TLR7 ELISA Kit allows for the in vitro quantitative determination of TLR7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Principle of the Assay: TLR7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TLR7 antibody and an TLR7-HRP conjugate. The assay sample and buffer are incubated together with TLR7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TLR7 concentration since TLR7 from samples and TLR7-HRP conjugate compete for the anti-TLR7 antibody binding site. Since the number of sites is limited, as more sites are occupied by TLR7 from the sample, fewer sites are left to bind TLR7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TLR7 concentration in each sample is interpolated from this standard curve.
  • Human Toll-like receptor 7 Protein information
  • Uniprot ID TLR7_HUMAN
  • Uniprot AC Q9NYK1; D1CS69; Q9NR98;
  • UniGene Hs.659215;
  • GeneID 51284
  • KEGG hsa:51284;
  • Human Toll-like receptor 7 Protein SEQUENCE
  • SEQUENCE 1049 AA; 120922 MW; 8C701E9E437F2721 CRC64;

    MVFPMWTLKR QILILFNIIL ISKLLGARWF PKTLPCDVTL DVPKNHVIVD

    CTDKHLTEIP GGIPTNTTNL TLTINHIPDI SPASFHRLDH LVEIDFRCNC

    VPIPLGSKNN MCIKRLQIKP RSFSGLTYLK SLYLDGNQLL EIPQGLPPSL

    QLLSLEANNI FSIRKENLTE LANIEILYLG QNCYYRNPCY VSYSIEKDAF

    LNLTKLKVLS LKDNNVTAVP TVLPSTLTEL YLYNNMIAKI QEDDFNNLNQ

    LQILDLSGNC PRCYNAPFPC APCKNNSPLQ IPVNAFDALT ELKVLRLHSN

    SLQHVPPRWF KNINKLQELD LSQNFLAKEI GDAKFLHFLP SLIQLDLSFN

    FELQVYRASM NLSQAFSSLK SLKILRIRGY VFKELKSFNL SPLHNLQNLE

    VLDLGTNFIK IANLSMFKQF KRLKVIDLSV NKISPSGDSS EVGFCSNART

    SVESYEPQVL EQLHYFRYDK YARSCRFKNK EASFMSVNES CYKYGQTLDL

    SKNSIFFVKS SDFQHLSFLK CLNLSGNLIS QTLNGSEFQP LAELRYLDFS

    NNRLDLLHST AFEELHKLEV LDISSNSHYF QSEGITHMLN FTKNLKVLQK

    LMMNDNDISS STSRTMESES LRTLEFRGNH LDVLWREGDN RYLQLFKNLL

    KLEELDISKN SLSFLPSGVF DGMPPNLKNL SLAKNGLKSF SWKKLQCLKN

    LETLDLSHNQ LTTVPERLSN CSRSLKNLIL KNNQIRSLTK YFLQDAFQLR

    YLDLSSNKIQ MIQKTSFPEN VLNNLKMLLL HHNRFLCTCD AVWFVWWVNH

    TEVTIPYLAT DVTCVGPGAH KGQSVISLDL YTCELDLTNL ILFSLSISVS

    LFLMVMMTAS HLYFWDVWYI YHFCKAKIKG YQRLISPDCC YDAFIVYDTK

    DPAVTEWVLA ELVAKLEDPR EKHFNLCLEE RDWLPGQPVL ENLSQSIQLS

    KKTVFVMTDK YAKTENFKIA FYLSHQRLMD EKVDVIILIF LEKPFQKSKF

    LQLRKRLCGS SVLEWPTNPQ AHPYFWQCLK NALATDNHVA YSQVFKETV

  • UCSC uc004cvc.4; human.;


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