Human TLR7 ELISA Kit

Alternative name
Human TLR7-like ELISA Kit;Human toll like receptor 7 ELISA Kit;Human toll-like receptor 7 ELISA Kit;Human toll-like receptor 7-like ELISA Kit;
Catalog
E038541
species
Human
GeneTLR7
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman TLR7 ELISA Kit allows for the in vitro quantitative determination of TLR7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalPrinciple of the Assay: TLR7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TLR7 antibody and an TLR7-HRP conjugate. The assay sample and buffer are incubated together with TLR7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TLR7 concentration since TLR7 from samples and TLR7-HRP conjugate compete for the anti-TLR7 antibody binding site. Since the number of sites is limited, as more sites are occupied by TLR7 from the sample, fewer sites are left to bind TLR7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TLR7 concentration in each sample is interpolated from this standard curve.
Human Toll-like receptor 7 Protein information
Uniprot ID TLR7_HUMAN
Uniprot AC Q9NYK1; D1CS69; Q9NR98;
UniGene Hs.659215;
GeneID 51284
KEGG hsa:51284;
Human Toll-like receptor 7 Protein SEQUENCE
SEQUENCE 1049 AA; 120922 MW; 8C701E9E437F2721 CRC64;
MVFPMWTLKR QILILFNIIL ISKLLGARWF PKTLPCDVTL DVPKNHVIVD
CTDKHLTEIP GGIPTNTTNL TLTINHIPDI SPASFHRLDH LVEIDFRCNC
VPIPLGSKNN MCIKRLQIKP RSFSGLTYLK SLYLDGNQLL EIPQGLPPSL
QLLSLEANNI FSIRKENLTE LANIEILYLG QNCYYRNPCY VSYSIEKDAF
LNLTKLKVLS LKDNNVTAVP TVLPSTLTEL YLYNNMIAKI QEDDFNNLNQ
LQILDLSGNC PRCYNAPFPC APCKNNSPLQ IPVNAFDALT ELKVLRLHSN
SLQHVPPRWF KNINKLQELD LSQNFLAKEI GDAKFLHFLP SLIQLDLSFN
FELQVYRASM NLSQAFSSLK SLKILRIRGY VFKELKSFNL SPLHNLQNLE
VLDLGTNFIK IANLSMFKQF KRLKVIDLSV NKISPSGDSS EVGFCSNART
SVESYEPQVL EQLHYFRYDK YARSCRFKNK EASFMSVNES CYKYGQTLDL
SKNSIFFVKS SDFQHLSFLK CLNLSGNLIS QTLNGSEFQP LAELRYLDFS
NNRLDLLHST AFEELHKLEV LDISSNSHYF QSEGITHMLN FTKNLKVLQK
LMMNDNDISS STSRTMESES LRTLEFRGNH LDVLWREGDN RYLQLFKNLL
KLEELDISKN SLSFLPSGVF DGMPPNLKNL SLAKNGLKSF SWKKLQCLKN
LETLDLSHNQ LTTVPERLSN CSRSLKNLIL KNNQIRSLTK YFLQDAFQLR
YLDLSSNKIQ MIQKTSFPEN VLNNLKMLLL HHNRFLCTCD AVWFVWWVNH
TEVTIPYLAT DVTCVGPGAH KGQSVISLDL YTCELDLTNL ILFSLSISVS
LFLMVMMTAS HLYFWDVWYI YHFCKAKIKG YQRLISPDCC YDAFIVYDTK
DPAVTEWVLA ELVAKLEDPR EKHFNLCLEE RDWLPGQPVL ENLSQSIQLS
KKTVFVMTDK YAKTENFKIA FYLSHQRLMD EKVDVIILIF LEKPFQKSKF
LQLRKRLCGS SVLEWPTNPQ AHPYFWQCLK NALATDNHVA YSQVFKETV
UCSC uc004cvc.4; human.;

Category

Human TLR7 ELISA Kit

Human TLR7-like ELISA Kit;Human toll like receptor 7 ELISA Kit;Human toll-like receptor 7 ELISA Kit;Human toll-like receptor 7-like ELISA Kit;