Human CD320 ELISA Kit

Alternative name
Human 8D6 antigen ELISA Kit;Human FDC-signaling molecule 8D6 ELISA Kit;Human FDC-SM-8D6 ELISA Kit;Human transcobalamin receptor ELISA Kit;Human TCBLR ELISA Kit;Human 8D6A ELISA Kit;Human 8D6 ELISA Kit;Human CD320 molecule ELISA Kit;Human CD320 antigen ELISA Kit;
Catalog
E007432
species
Human
GeneCD320
SpecificityNatural and recombinant human CD320
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman CD320 ELISA Kit allows for the in vitro quantitative determination of CD320 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human CD320 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD320 has been precoated onto 96-well plates. Standards(NSO, S36-Y229) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD320 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD320 amount of sample captured in plate. Background: CD320(cluster of differentiation 320), also known as 8D6A or TCBLR, is a human gene. It is mapped to chromosome 19p13.2. This gene encodes the transcobalamin receptor that is expressed at the cell surface. It mediates the cellular uptake of transcobalamin bound cobalamin (vitamin B12), and the expression of CD320 enhanced B-cell proliferation and immunoglobulin secretion. Mutations in this gene are associated with methylmalonic aciduria. It has been found that antagonists of the CD320 signaling pathway may counter the growth of follicular lymphomas or other tumors that metastasize to lymphoid follicles. CD320 is also a specific receptor for uptake of TCN2-bound cobalamin.
Human CD320 antigen Protein information
Uniprot ID CD320_HUMAN
Uniprot AC Q9NPF0; B2RDS5; D6W668; F5H6D3; Q53HF7;
UniGene Hs.558499;
GeneID 51293
KEGG hsa:51293;
Human CD320 antigen Protein SEQUENCE
SEQUENCE 282 AA; 28991 MW; 59E172986B220E4F CRC64;
MSGGWMAQVG AWRTGALGLA LLLLLGLGLG LEAAASPLST PTSAQAAGPS
SGSCPPTKFQ CRTSGLCVPL TWRCDRDLDC SDGSDEEECR IEPCTQKGQC
PPPPGLPCPC TGVSDCSGGT DKKLRNCSRL ACLAGELRCT LSDDCIPLTW
RCDGHPDCPD SSDELGCGTN EILPEGDATT MGPPVTLESV TSLRNATTMG
PPVTLESVPS VGNATSSSAG DQSGSPTAYG VIAAAAVLSA SLVTATLLLL
SWLRAQERLR PLGLLVAMKE SLLLSEQKTS LP
UCSC uc002mjj.3; human. [Q9NPF0-1];

Category

Human CD320 ELISA Kit

Human 8D6 antigen ELISA Kit;Human FDC-signaling molecule 8D6 ELISA Kit;Human FDC-SM-8D6 ELISA Kit;Human transcobalamin receptor ELISA Kit;Human TCBLR ELISA Kit;Human 8D6A ELISA Kit;Human 8D6 ELISA Kit;Human CD320 molecule ELISA Kit;Human CD320 antigen ELISA Kit;